Laboratory science
Mitomycin C dissolved in a reversible thermosetting gel: target
tissue concentrations in the rabbit eye
Department
of Ophthalmology, Gifu University School of Medicine, Gifu City, Japan
Correspondence to: Tetsuya Yamamoto, MD, Department of Ophthalmology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-shi, Japan, 500.
Accepted for publication 14 October 1996
AIMS
To determine whether a new, reversible
thermosetting gel enhances mitomycin C transfer to target ocular
tissues in the rabbit eye.
METHODSA 0.1 ml solution of mitomycin C
containing 0.22 µg, 2.9 µg, or 28 µg of the agent dissolved in a
reversible thermosetting gel consisting of methylcellulose, citric
acid, and polyethylene glycol was injected subconjunctivally in 30 New
Zealand albino rabbits. Scleral and conjunctival tissues were excised
at 0.5, 1, 2, 4, or 24 hours after the injection and mitomycin C
concentrations in these tissues were determined by high performance
liquid chromatography. The concentration over time was approximated to
a single exponential curve, and initial mitomycin C concentrations,
time constants, and half life values were determined. Finally, the
areas under the curves (AUCs) between 0.5 and 24 hours were calculated.
RESULTSThe mitomycin C concentrations in the
target tissues were dose dependent and decreased rapidly over 24 hours.
Both the initial mitomycin C concentrations as well as AUCs in these
eyes treated with mitomycin C, dissolved in a reversible thermosetting
gel, were higher than those in eyes treated similarly in a previous study in which the gel was not used.
CONCLUSIONApplied subconjunctivally in the
rabbit eye, mitomycin C dissolved in the reversible thermosetting gel
enhanced transfer of the agent to the sclera and the conjunctiva.
© 1997 by British Journal of Ophthalmology
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