Laboratory science
Pituitary adenylate cyclase activating polypeptide and nitric
oxide synthase are expressed in the rat ciliary ganglion
a Department of Ophthalmology, Trondheim, Norway, b Department of Oto-Rhino-Laryngology, Malmö
General Hospital, Malmö, Sweden, c Department
of Physiology and Neuroscience, Section for Neuroendocrine Cell
Biology, University of Lund, Sweden
Correspondence to: Tor Elsås, Department of Ophthalmology, University Hospital of Trondheim, N-7006, Trondheim, Norway.
Accepted for publication 25 November
1996
AIMS
To study the distribution in the rat ciliary
ganglion of neurons synthesising and storing the recently discovered
neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP)
and neuronal nitric oxide synthase (NOS), the neuronal marker of the novel gaseous transmitter nitric oxide.
METHODSNeurons expressing PACAP and neuronal NOS
mRNA were identified in the rat ciliary ganglion by in situ
hybridisation with radiolabelled oligonucleotide probes.
Immunocytochemistry was used to demonstrate immunoreactive
neuropeptides and NOS.
RESULTSImmunocytochemistry demonstrated
immunoreactivity for PACAP and NOS in a small number of neuronal cell
bodies. In situ hybridisation revealed that NOS and PACAP were
expressed in numerous ganglion cell somata. The well established
ciliary messengers vasoactive intestinal peptide and neuropeptide Y
were found in a large number of neuronal cell bodies.
CONCLUSIONThese results demonstrate that PACAP
and NOS are synthesised and stored in the ciliary ganglion. These
findings further illustrate the mixed nature of the ciliary ganglion
and may provide a basis for the understanding of the diverse
physiological functions of this ganglion.
© 1997 by British Journal of Ophthalmology
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