Laboratory science
Tenascin-C expression in normal, inflamed, and scarred human
corneas
a Department of Pathological Sciences, University of
Manchester, b Department
of Ophthalmology, Manchester Royal Eye Hospital
Correspondence to: Dr Richard E Bonshek, Department of Ophthalmology, University of Manchester, Royal Eye Hospital, Oxford Road, Manchester M13 9WH.
Accepted for publication 10 April 1997
AIMS/BACKGROUND
In adult tissues the expression of
tenascin-cytotactin (TN-C), an extracellular matrix glycoprotein, is
limited to tumours and regions of continuous renewal. It is also
transiently expressed in cutaneous and corneal wound healing. There are
limited data regarding its expression in inflammation and scarring of
the adult human cornea. In this study, TN-C expression patterns in
normal, inflamed, and scarred human corneas have been examined.
METHODSPenetrating keratoplasty specimens were
selected from cases of herpes simplex keratitis, herpes zoster
ophthalmicus, rheumatoid arthritis ulceration, bacterial keratitis,
rosacea keratitis, interstitial keratitis, and previous surgery so as
to encompass varying degrees of active and chronic inflammation and
scarring. TN-C in these and in normal corneas was immunodetected using
TN2, a monoclonal antibody to human TN-C.
RESULTSThere was no TN2 immunopositivity in
normal corneas except at the corneoscleral interface. In pathological
corneas, TN2 immunopositivity was localised in and around regions of
active inflammation, fibrosis, and neovascularisation. TN2 positivity
was less in acute inflammation than in active chronic inflammation.
Mature, avascular scar tissue and epithelial downgrowth were TN2 negative.
CONCLUSIONThese results indicate that in the
adult human cornea, TN-C expression is induced in regions of
inflammation, fibrosis, and neovascularisation, but that expression is
absent in mature, avascular scar tissue. This suggests a role for this
glycoprotein in inflammation, healing, and extracellular matrix
reorganisation of the cornea.
© 1997 by British Journal of Ophthalmology
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