Interferon gamma immunoreactivity in iris nerve fibres during endotoxin induced uveitis in the rat
a Netherlands Ophthalmic Research Institute, Amsterdam,
Netherlands, b Zhongshan Ophthalmic Centre, Guangzhou, PR China, c University of Amsterdam, Netherlands
Correspondence to: Prof dr A Kijlstra, Netherlands Ophthalmic Research Institute, PO Box 12141, 1100 AC Amsterdam, Netherlands.
Accepted for publication 9
January 1998
AIMS
Previous studies have implied that interferon
gamma (IFN-
) is involved in the pathogenesis of endotoxin induced
uveitis (EIU) in the rat. This study investigated the source of IFN-
in the iris during EIU.
METHODSWhole mounts of iris were isolated from
Lewis rats before and at different times (from 4 hours to 14 days)
after foot pad injection of 200 µg Salmonella
typhimurium lipopolysaccharide (LPS). Immunohistological
analysis was performed using monoclonal antibodies (mAbs) specific to
rat IFN- (DB12 and DB13). mAbs specific to monocytes, macrophages,
and dendritic cells and MHC class II were used to asses the
inflammatory response in the eye (ED-1, ED-2, and OX-6). An antibody
specific to neurofilaments (2H3) was used to stain nerve fibres in the
normal iris.
RESULTSLPS administration induced acute
intraocular inflammation, characterised by a massive infiltration of
monocytes/macrophages and increased numbers of MHC class II positive
cells in the iris. IFN- immunoreactive cells were not detected in
iris whole mounts of control rats. Strikingly, IFN-
immunoreactivity was found in fibres from 4 hours until 10 days
after LPS injection, with the most intense staining at 48-72 hours.
Other DB12 or DB13 positive cells were not detected in the iris. The
pattern of DB12 and DB13 staining in the inflamed iris was similar to
the 2H3 staining of neurons in the iris of control rats.
CONCLUSIONThese results show that systemic LPS
administration induces IFN- immunoreactivity in iris fibres and
suggest that iris nerve fibres may be a source of IFN- during EIU.
The IFN- immunoreactive material in the iris nerve fibres may be
identical to neuronal IFN-.
© 1998 by British Journal of Ophthalmology
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
-
Chang, Y.-H., Horng, C.-T., Chen, Y.-H., Chen, P.-L., Chen, C.-L., Liang, C.-M., Chien, M.-W., Chen, J.-T.
(2008). Inhibitory Effects of Glucosamine on Endotoxin-Induced Uveitis in Lewis Rats. IOVS
49: 5441-5449
[Abstract] [Full Text] -
Wax, M. B., Tezel, G., Yang, J., Peng, G., Patil, R. V., Agarwal, N., Sappington, R. M., Calkins, D. J.
(2008). Induced Autoimmunity to Heat Shock Proteins Elicits Glaucomatous Loss of Retinal Ganglion Cell Neurons via Activated T-Cell-Derived Fas-Ligand. J. Neurosci.
28: 12085-12096
[Abstract] [Full Text] -
McMenamin, P. G.
(2000). Optimal Methods for Preparation and Immunostaining of Iris, Ciliary Body, and Choroidal Wholemounts. IOVS
41: 3043-3048
[Abstract] [Full Text]
- Full Text
- Full Text (PDF)
- Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
- Citation Map
Services
- Email this link to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
PubMed
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
