Interphase fluorescence in situ hybridisation of the X and Y chromosomes in the human eye
Gregor Wollensaka, Elizabeth J Perlmanb, W Richard Greena
a Eye Pathology
Laboratory, Wilmer Eye Institute, Johns Hopkins University Medical
Institutions, Baltimore, MD, USA, b Department of Pathology, Johns Hopkins
University Medical Institutions, Baltimore, MD, USA
Correspondence to: Dr G Wollensak, Wildentensteig 4, D-14195 Berlin, Germany gwollens{at}hotmail.com
Accepted for publication 14 May 2001
AIM
To determine the
sex of individual cells in paraffin sections of the human eye by
fluorescence in situ hybridisation (FISH) of the X and Y chromosomes.
METHODSThe authors
developed a protocol for FISH of the X and Y chromosomes in paraffin
sections of human eyes.
RESULTSIn all the
specimens that had been fixed in 10% formalin and with a fixation time
of up to 3 days sex determination of individual cells was achieved. The
percentage of cells with clearly identifiable signals was up to 98%
for corneal epithelium, keratocytes, corneal endothelium, trabecular
meshwork, lens epithelium, retina, and optic nerve.
CONCLUSIONSFISH
allows the determination of the sex of single cells in paraffin
sections of human eyes without destruction of the tissue structure. Its
main application is the histological analysis of sex mismatched
corneal, RPE, or neuroretinal transplants to distinguish host and donor cells.
© 2001 by British Journal of Ophthalmology
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