Influence of HLA-A, HLA-B, and HLA-DR matching on rejection of random corneal grafts using corneal tissue for retrospective DNA HLA typing
Marjolijn C Bartelsa, Henderikus G Ottenb, B Elske van Gelderenc, Allegonda Van der Lelijd
a Department of
Ophthalmology, University Hospital Rotterdam, Netherlands, b Department of Medical Immunology,
University Medical Centre Utrecht, Netherlands, c Department of Ophthalmo-Immunology, The
Netherlands Ophthalmic Research Institute, Amsterdam, Netherlands, d Department
of Ophthalmology, Leiden University Medical Centre, Netherlands
Correspondence to: Dr M C Bartels, Department of Ophthalmology, University Hospital Rotterdam, PO Box 2040, 3000 CA Rotterdam, Netherlands bartels{at}oogk.azr.nl
Accepted for publication 23 May 2001
AIM
To establish if
coincidental HLA-A, HLA-B, and HLA-DR tissue matching is associated
with a reduced likelihood of corneal graft rejection.
METHODSOrgan culture
preserved random donor corneas were used for penetrating keratoplasty
(PKP). Corneal tissue from all graft recipients and donors or blood
samples from recipients after repeated transplantation were obtained in
order to perform retrospective molecular HLA typing. A group of 21 recipients with a rejection episode (cases) after corneal
transplantation was compared with a control group of non-rejectors (n = 43). 31 graft recipients were considered as high risk patients. The
influence of HLA-A, HLA-B, and HLA-DR matching on rejection free graft
survival time was analysed with Kaplan-Meyer statistics and Cox regression.
RESULTSA prolonged
rejection free survival time was observed in graft recipients with one
or two HLA-A matches (log rank test, p = 0.034). This effect was also
observed in high risk graft recipients with one or two HLA-DR matches
(log rank test, p = 0.030).
CONCLUSIONSCoincidental
HLA-A and HLA-DR matches were observed and associated with a prolonged
rejection free survival time in the total group and in the high risk
group, respectively. These results support the beneficial effect of
prospective HLA-A and HLA-DR typing upon corneal graft survival.
© 2001 by British Journal of Ophthalmology
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
- Full Text
- Full Text (PDF)
- Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
- Citation Map
Services
- Email this link to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
PubMed
Topic Collections
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
