Latent TGF
binding protein-1 and fibrillin-1 in human capsular
opacification and in cultured lens epithelial cells
Shizuya Saikaa, Takeshi Miyamotoa, Takeshi Tanakaa, Iku Ishidaa, Yoshitaka Ohnishia, Akira Ooshimab
a Department of
Ophthalmology, Wakayama Medical University School of Medicine,
Wakayama, Japan, b Department of
Pathology
Correspondence to: Shizuya Saika, MD, Department of Ophthalmology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama, 641-8509, Japan shizuya{at}wakayama-med.ac.jp
Accepted for publication 10 May 2001
BACKGROUND/AIM
It was
previously reported that collagenous extracellular matrix (ECM) in
human capsular opacification contained isoforms of transforming growth
factor 
(TGF). In the present study, the authors performed
immunohistochemistry to examine whether ECM in human capsular
opacification and in cultures of bovine lens epithelial cells (LECs)
contained latent TGF binding protein-1 (LTBP-1), TGF1 latency
associated peptide (1-LAP), and fibrillin-1, a suspected ligand of
LTBP-1 as well as a component of the extracellular microfibrillar
apparatus. The aim of the study was to further clarify the mechanism of
TGF1 deposition in ECM of capsular opacification.
METHODSHuman capsular
opacification specimens and uninjured lens capsules, as well as
cultured bovine LECs, were processed for immunohistochemistry using
antibodies against LTBP-1, 1-LAP, fibrillin-1, and collagen type I.
RESULTSLTBP-1,
1-LAP, and fibrillin-1 all were localised to the ECM in human
capsular opacification. Uninjured lens epithelium stained for 1-LAP,
but not for LTBP-1 and fibrillin-1. ECM deposited in confluent LEC
cultures stained for LTBP-1, 1-LAP, and fibrillin-1, while cultures
with only sparse cellularity were unstained for LTBP-1 or fibrillin-1.
CONCLUSIONSLECs
upregulate LTBP-1 and fibrillin-1 during postoperative healing. LTBP-1,
1-LAP, and fibrillin-1 colocalised to the ECM in capsular
opacification and in confluent LEC cultures. TGF1 is considered to
deposit in ECM in the large latent form. ECM secreted by LEC may
function as a scavenger or repository of TGF.
© 2001 by British Journal of Ophthalmology
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