LABORATORY SCIENCE

DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture

N Eter and M Spitznas

Department of Ophthalmology, University of Bonn Medical Center, Bonn, Germany

Correspondence to:
Correspondence to:
Nicole Eter, MD, Department of Ophthalmology, University of Bonn Medical Center, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany;
eter{at}uni-bonn.de

Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation.

Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 µg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein.

Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well.

Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone.

Keywords: choriocapillary endothelial cells; thalidomide; DMSO; cell proliferation

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