CLINICAL SCIENCE

Double vital staining using trypan blue and infracyanine green in macular pucker surgery

P Stalmans¹, E J Feron², R Parys-Van Ginderdeuren¹, A Van Lommel³, G R J Melles⁴ and M Veckeneer²

¹ Department of Ophthalmology, UZLeuven, Belgium
² Eye Hospital, Rotterdam, Netherlands
³ Department of Pathology, UZLeuven, Belgium
⁴ Netherlands Institute for Innovative Ocular Surgery, Rotterdam, Netherlands

Correspondence to:
Correspondence to:
Peter Stalmans, MD, PhD, Department of Ophthalmology, UZLeuven, Capucijnenvoer 33, B3000 Leuven, Belgium;
Peter.Stalmans{at}uz.kuleuven.ac.be

Aims: To study the clinical properties of double vital staining in premacular fibrosis, facilitating complete removal of all epiretinal tissue.

Methods: In a two step surgery, the epiretinal pucker was removed after staining with trypan blue, whereafter the inner limiting membrane was peeled after staining with infracyanine green.

Results: In all 30 patients, a separate epiretinal layer and inner limiting membrane were removed from the macular area. Pathological examination showed different histological properties of the removed layers. An increased visual acuity was measured in 26 patients, and a slightly decreased visual acuity in one patient.

Conclusion: The described double staining technique could be a novel valuable tool that may help to achieve optimal anatomical and functional recovery after surgery for premacular fibrosis

Keywords: vital staining; infracyanine green; trypan blue; epiretinal membranes; pucker; inner limiting membrane

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