Article Text
Abstract
Aims: To search for the induction of the expression of antimicrobial peptides in corneal fibroblasts treated with bacterial components.
Methods: RT-PCR was performed to search for mRNAs expression of antimicrobial peptides and toll-like receptors (TLRs) in murine primary cultures of corneal fibroblast (PCCF) treated with lipopolysaccharide (LPS) from Escherichia coli, peptidoglycan from Staphylococcus aureus, and cytosine-phosphorous-guanine oligonucleotide (CpG-ODN). Cellular activation was blocked with anti-TRL antibodies.
Results: LPS did not induce expression of antimicrobial peptide in corneal fibroblasts. Cathelin related antimicrobial peptide (CRAMP) and α-defensin 3 were overexpressed in a time and dose dependent manner in corneal fibroblasts treated with peptidoglycan and with CpG-ODN, respectively. CRAMP expression was blocked when PCCF were treated with anti-TLR-2 antibodies. α-Defensin 3 was not expressed in NIH murine corneal fibroblasts (which do not express the TLR-9 molecule) treated with CpG-ODN.
Conclusion: Results suggest that corneal fibroblasts, which are the second cellular barrier of the cornea, can play an important part in the innate immunity of the eye via TLR stimulation.
- AD-3, α-defensin 3
- BD-1, β-defensin-1
- CpG-ODN, cytosine-phosphorous-guanine oligonucleotide
- CRAMP, cathelin related antimicrobial peptide
- HNP, human neutrophil peptides
- LPS, lipopolysaccharide
- NF-kB, nuclear factor kappa B
- PAMPs, pathogen associated molecular patterns
- PCCF, primary cultures of corneal fibroblast
- PCR, polymerase chain reaction
- PGNs, peptidoglycans
- RT, reverse transcriptase
- TIR, TLR/IL-1 receptor
- TLRs, toll-like receptors
- TNF, tumour necrosis factor
- CRAMP
- α-defensin 3
- corneal fibroblast
- toll-like receptors
- antimicrobial peptides
- AD-3, α-defensin 3
- BD-1, β-defensin-1
- CpG-ODN, cytosine-phosphorous-guanine oligonucleotide
- CRAMP, cathelin related antimicrobial peptide
- HNP, human neutrophil peptides
- LPS, lipopolysaccharide
- NF-kB, nuclear factor kappa B
- PAMPs, pathogen associated molecular patterns
- PCCF, primary cultures of corneal fibroblast
- PCR, polymerase chain reaction
- PGNs, peptidoglycans
- RT, reverse transcriptase
- TIR, TLR/IL-1 receptor
- TLRs, toll-like receptors
- TNF, tumour necrosis factor
- CRAMP
- α-defensin 3
- corneal fibroblast
- toll-like receptors
- antimicrobial peptides
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- AD-3, α-defensin 3
- BD-1, β-defensin-1
- CpG-ODN, cytosine-phosphorous-guanine oligonucleotide
- CRAMP, cathelin related antimicrobial peptide
- HNP, human neutrophil peptides
- LPS, lipopolysaccharide
- NF-kB, nuclear factor kappa B
- PAMPs, pathogen associated molecular patterns
- PCCF, primary cultures of corneal fibroblast
- PCR, polymerase chain reaction
- PGNs, peptidoglycans
- RT, reverse transcriptase
- TIR, TLR/IL-1 receptor
- TLRs, toll-like receptors
- TNF, tumour necrosis factor
- CRAMP
- α-defensin 3
- corneal fibroblast
- toll-like receptors
- antimicrobial peptides
- AD-3, α-defensin 3
- BD-1, β-defensin-1
- CpG-ODN, cytosine-phosphorous-guanine oligonucleotide
- CRAMP, cathelin related antimicrobial peptide
- HNP, human neutrophil peptides
- LPS, lipopolysaccharide
- NF-kB, nuclear factor kappa B
- PAMPs, pathogen associated molecular patterns
- PCCF, primary cultures of corneal fibroblast
- PCR, polymerase chain reaction
- PGNs, peptidoglycans
- RT, reverse transcriptase
- TIR, TLR/IL-1 receptor
- TLRs, toll-like receptors
- TNF, tumour necrosis factor
- CRAMP
- α-defensin 3
- corneal fibroblast
- toll-like receptors
- antimicrobial peptides