| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
|
|
|
|
|||||||||||||
|
|
|||||||||||||||
|
Published Online First: 1 February 2008. doi:10.1136/bjo.2007.125773 British Journal of Ophthalmology 2008;92:505-508 Copyright © 2008 by the BMJ Publishing Group Ltd.
Association of varicella zoster virus load in the aqueous humor with clinical manifestations of anterior uveitis in herpes zoster ophthalmicus and zoster sine herpeteS Kido1, S Sugita1, S Horie1, M Miyanaga2, K Miyata2, N Shimizu3, T Morio4, M Mochizuki1
1 Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University, Tokyo, Japan
Correspondence to: Accepted 4 August 2007
Aim: To investigative whether clinical manifestations of anterior uveitis are associated with the viral load of varicella zoster virus (VZV) in the aqueous humor in patients with herpes zoster ophthalmicus (HZO) and zoster sine herpete (ZSH). Methods: After informed consent was given, an aliquot of aqueous humor was collected from patients with VZV anterior uveitis (n = 8). Genomic DNA of the human herpes viruses was measured in the aqueous humor by two PCR assays: a qualitative multiplex PCR and a quantitative real-time PCR. Results: All patients had unilateral acute anterior uveitis with high intraocular pressure, mutton fat keratic precipitates with some pigmentation, and trabecular meshwork pigmentation. Multiplex PCR demonstrated VZV genomic DNA in all of the samples, but not in other human herpes virus samples (human simplex virus types 1 and 2, Epstein–Barr virus, cytomegalovirus and human herpes virus types 6, 7 and 8). Real-time PCR revealed a high copy number of VZV DNA in the aqueous humor. After the initial onset of anterior uveitis, iris atrophy and distorted pupil with paralytic mydriasis developed. The intensity of iris atrophy and pupil distortion, but not ocular hypertension, correlated with the viral load of VZV in the aqueous humor. Conclusion: VZV viral load in the aqueous humor correlated significantly with damage to the iris (iris atrophy and pupil distortion) in patients with HZO and ZSH.
Varicella zoster virus (VZV) affects the first branch of the trigeminal nerve and is known to cause unilateral anterior uveitis (VZV anterior uveitis) characterised by mutton-fat keratic precipitates (KPs), trabecular meshwork pigmentation, ocular hypertension, iris atrophy and distorted pupil. Systemic signs in VZV iridocyclitis can be herpes zoster ophthalmicus (HZO) with skin eruptions or zoster sine herpete (ZSH) without skin eruptions but solely with neuralgia. Using PCR, previous studies have revealed genomic DNA of VZV in the aqueous humor in patients with anterior uveitis with HZO and ZSH.1 2 Recent advances in molecular biology now make it possible for quantitative measurement of the viral load using real-time PCR. Therefore, this study aimed to quantitatively measure the viral load of VZV in the aqueous humor and analyse the correlation between viral load in the aqueous humor and clinical manifestations of VZV anterior uveitis in patients with HZO and ZSH.
Subjects The subjects were eight patients (three men and five women; age range 43–71 years (mean 61)) with diagnosed VZV anterior uveitis at Tokyo Medical and Dental University Hospital and Miyata Hospital between December 1999 and September 2007. The clinical diagnosis of VZV anterior uveitis was based on observation of anterior uveitis associated with either HZO or ZSH. After informed consent had been obtained, an aliquot of aqueous humor (0.1–0.2 ml) was obtained from each patient. The research followed the tenets of the Declaration of Helsinki, and the study was approved by the institutional ethics committees of Tokyo Medical and Dental University.
PCR assay Real-time PCR was performed only for HHV, the genomic DNA of which was detected by multiplex PCR. It was performed by using Amplitaq Gold and a Real-Time PCR 7300 system (ABI, Forster City, CA, USA). The primer sequences for VZV (ORF29) used in real-time PCR were designed to use Primer Express (ABI): forward, AACTTTTACATCCAGCCTGGCG; reverse, GAAAACCCAAACCGTTCTCGAG. The probe was FAM-TGTCTTTCACGGAGGCAAACACGT-TAMRA. The following PCR conditions were used: denaturation at 95°C for 10 min, 95°C for 15 s, and 60°C for 60 s for 40 cycles.
Statistical analysis
Clinical manifestations In four of the patients, there was an episode of skin eruption with neuralgia in the area of the first branch of the trigeminal nerve; this was clinically diagnosed as HZO (table 1). No skin eruptions were observed in the other patients, although they complained of pain near the first branch of the trigeminal nerve, leading to the diagnosis of ZSH. At various intervals after the onset of HZO or ZSH, the patients developed unilateral anterior uveitis. All patients had cells and flare in the anterior chamber, with increased flare values measured by a laser flare meter (Cowa, Tokyo, Japan). Four patients exhibited mutton-fat KPs (table 1), which had brownish pigmentation and were small or medium in size. Three patients with HZO already exhibited iris atrophy and pupil distortion on referral. High intraocular pressure (IOP) was recorded in all patients except in case 3 (table 1). A gonioscopic examination revealed wide, open angles in all patients, and higher pigmentation in the affected eye than the other eye in six of the eight patients. Ophthalmoscopic examinations revealed no significant inflammatory lesions in the retina and choroid. These clinical results led us to a diagnosis of anterior uveitis associated with HZO or ZSH, with paracentesis subsequently performed in order to carry out the PCR analysis.
After confirmation of the presence of VZV in the aqueous humor by multiplex PCR, systemic anti-VZV agents (aciclovir or valaciclovir) and aciclovir ointment were administered for at least 4 weeks together with a topical corticosteroid (eg, betamethasone) and anti-glaucoma agents (eg, timolol and latanoprost). Iris atrophy and pupil distortion developed during the clinical course (fig 1, table 2). The anterior uveitis and high IOP responded well to treatment.
PCR analysis of the aqueous humor Qualitative PCR (multiplex PCR) detected genomic DNA of VZV but not of other human herpes viruses (HSV-1, HSV-2, EBV, CMV, HHV-6, HHV-7 and HHV-8) in the aqueous humor of all eight patients (table 1). In peripheral blood samples, however, no genomic DNA of any of the eight human herpes viruses, including VZV, was detected in any of the patients.
Quantitative PCR (real-time PCR) detected significant viral loads of VZV DNA in the aqueous humor of the eight patients ranging from 3.8x102 to 1.2x107 copies/ml (table 2). It is of note that the VZV viral load in the aqueous humor correlated with the intensity of iris atrophy and pupil distortion (table 2, fig 1). Patient 1 had the highest VZV viral load (1.2x107 copies/ml) in the aqueous humor and also had the most severe iris atrophy, with multiple wide areas of segmental iris atrophy and a widely dilated pupil (fig 1A, table 2). Patients 2–5 had the second highest viral loads in the aqueous humor ( We next examined whether higher viral load in the aqueous humor was significantly associated with the intensity of the iris atrophy and pupil distortion. For this analysis, we separated the patients into two groups: higher viral load (patients 1–5) and lower viral load (patients 6–8). The extent of iris atrophy was significantly (p<0.05) larger in the group with higher viral load. In addition, the maximum pupil diameter in the pupil distortion was significantly (p<0.05) larger in the group with higher viral load. These results suggest that higher viral load in the aqueous humor is closely associated with the intensity of the iris atrophy and pupil distortion.
Human herpes viruses are known to be involved in many ocular pathological conditions, such as keratitis, anterior uveitis and necrotising retinitis. Early treatment with anti-viral agents, based on a rapid and accurate diagnosis of the viral infection in ocular tissues, is clinically important in order to avoid the irreversible tissue damage and visual impairment caused by viral infection. Recent advances in PCR methodology have made it possible to screen for viral infection and further quantify the intensity of the viral infection in ocular inflammatory diseases.1–3 5–8 Asano et al8 detected herpes virus DNA (VZV or HSV-2) in three cases of acute retinal necrosis using real-time PCR. They monitored viral load in ocular samples of these patients and examined correlations with disease activities of acute retinal necrosis. In this study, we used multiplex PCR to screen for HHV infections (HHV1–8) and real-time PCR to quantify viral load in the aqueous humor of patients with HZO or ZSH. With multiplex PCR, genomic DNA of VZV was detected in the aqueous humor of all eight patients with HZO and ZSH, and significant VZV viral loads were quantified in the same samples with real-time PCR. After cutaneous lesions or neuralgia, all eight patients with HZO or ZSH developed anterior uveitis, which was characterised by unilateral acute anterior uveitis with high IOP, mutton-fat KPs, trabecular meshwork pigmentation, iris atrophy and pupil distortion, although there were no significant pathological lesions in the cornea or ocular fundus of any of the patients. It is well established that these ocular signs are typical of HZO. The present multiplex PCR data clearly confirm that VZV, but not the other human herpes viruses, is responsible for the anterior uveitis. Furthermore, an important finding in our study was that the extent of iris atrophy and pupil distortion also correlated with the viral load of VZV in the aqueous humor in patients with HZO and ZSH. Unlike iris atrophy, IOP did not correlate with the viral load in the aqueous humor. All but one of the patients had raised IOP ranging from 22 to 46 mm Hg at the onset of anterior uveitis. This study did not reveal any pathological mechanisms for the correlation of the high VZV viral load with the iris atrophy. However, a previous immunohistological report detected VZV antigens in the stroma and vascular endothelial cells of the iris in anterior uveitis patients with HZO.9 Another study that used angiography in a patient with HZO showed that there was occlusion of the blood vessels of the atrophic iris.10 On the basis of previous studies and our present data, we hypothesise that the pathological changes caused by VZV may induce occlusion of the iris vessel leading to iris muscle paralysis, with a net result of iris atrophy and pupil distortion. The higher the viral load in the anterior chamber, the more VZVs there will be in the iris, and thus the more intense the pathological tissue damage. Early initiation of systemic anti-viral agents, as guided by PCR analysis, would help to avoid, or at least minimise, tissue damage in the iris. Some patients with HZO were only given 1-week systemic anti-viral treatments after the onset of the skin lesions. In these patients, the viral loads of VZV in the aqueous humor were high, and various degrees of iris atrophy and distorted pupil were seen over time. These observations suggest that systemic anti-viral treatments after the onset of HZO are helpful in avoiding or minimising irreversible ocular complications and that qualitative and quantitative PCR can also provide useful information for developing treatments for such patients.
We thank Drs Ken Watanabe and Miki Mizukami for technical assistance. This work was supported by a Grant-in-Aid for Young Scientists (B) 18791263 from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Competing interests: None declared. Ethics approval: Ethics approval was obtained. Patient consent: Consent has been obtained for publication of fig 1. Published Online First 1 February 2008
This article has been cited by other articles:
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
TOP
ABSTRACT
MATERIALS AND METHODS
1x106 copies/ml), and these patients developed multiple segmental or circular iris atrophy with moderate pupil distortion, although these signs were not as marked as in patient 1 (
