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Expression and clinical significance of connective tissue growth factor (CTGF) in Graves' ophthalmopathy
  1. Yi-Ming Huang1,
  2. Pei-Chen Chang2,
  3. Shi-Bei Wu3,
  4. Hui-Chuan Kau1,4,
  5. Chieh-Chih Tsai1,
  6. Catherine Jui-Ling Liu1,
  7. Yau-Huei Wei2,5
  1. 1Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan
  2. 2Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan
  3. 3Department of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan
  4. 4Department of Ophthalmology, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan
  5. 5Department of Medicine and Institute of Biomedical Sciences, Mackay Medical College, New Taipei City, Taiwan
  1. Correspondence to Dr Chieh-Chih Tsai, Department of Ophthalmology, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei, Taiwan; cctsai{at}vghtpe.gov.tw

Abstract

Aims To examine the expression of connective tissue growth factor (CTGF) in human cultured orbital fibroblasts from patients with Graves' ophthalmopathy (GO) and investigate whether a correlation exists between the presence of CTGF protein and clinical parameters of the disease.

Methods The protein expression levels of CTGF were analysed by western blots in cultured orbital fibroblasts from 10 patients with GO and 7 age-matched normal controls. Associations between the protein expression of CTGF and the clinical factors of GO, including clinical demographics, thyroid function, clinical activity score (CAS) and ophthalmopathy index (OI), was evaluated.

Results The mean protein expression levels of CTGF in the GO orbital fibroblasts were significantly higher than those of normal controls (p<0.001). Based on further analysis, the protein expression levels of CTGF in the GO orbital fibroblasts had significant correlation with gender (p=0.029), serum levels of thyrotropin receptor antibodies (p=0.029), CAS (p=0.048) and OI (p=0.043). Especially, there was a significant correlation between protein expression levels of CTGF and lid oedema (p=0.037), proptosis (p=0.045) and corneal involvement (p=0.001).

Conclusions Our findings revealed that the protein expression levels of CTGF in the GO orbital fibroblasts were significantly highly expressed than those of normal controls, and the elevated CTGF was associated with clinical characteristics and evolution, indicating CTGF may play a role in the pathogenesis and pathophysiology of GO.

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