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Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction.
  1. M. J. Taylor and
  2. C. J. Hunt

    Abstract

    Evaluation of corneal endothelial integrity by combined staining with the vital stain trypan blue and the intercellular stain alizarin red S provides a simple, quick technique for visualisation of both damaged and normal cells, thereby permitting the quantification of endothelial cell damage. Adjustment of the pH of the alizarin red S reagent to 4.2 is important for optimum dye-laking at the intercellular borders, and brief fixation with glutaraldehyde maintains the staining effect of both dyes.

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