Tear and serum IgG, IgA, and IgM levels were measured in 35 healthy subjects and 31 patients with acute conjunctivitis due to adenovirus 2, 7, and 8 infections. Serum IgG and IgM levels were found to be significantly low and there was no change in the serum IgA level. There was a significant rise in the tear IgG level. A progressive decline in the serum immunoglobulin level with the severity of the disease was observed. The changes in the immunoglobulin levels in serum and tears showed a tendency to reach normal levels with clinical improvement.