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Br J Ophthalmol 1997;81:391-395 doi:10.1136/bjo.81.5.391
  • Original Article
    • Laboratory science

Influence of topical human epidermal growth factor on postkeratoplasty re-epithelialisation

  1. M M M J Dellaerta,
  2. T A Caseyb,
  3. S Wiffenb,
  4. J Gordonc,
  5. P Johnsonc,
  6. A J M Geerardsa,
  7. W J Rijnevelda,
  8. L Remeijera,
  9. P G H Mulderd,
  10. W H Beekhuisa
  1. aCornea Service, Rotterdam Eye Hospital, Rotterdam, the Netherlands, bCorneo-plastic Unit, Queen Victoria Hospital, East Grinstead, UK, cChiron Vision, Claremont, California, USA, dDepartment of Epidemiology and Biostatistics, Erasmus University, Rotterdam, the Netherlands
  1. W Houdijn Beekhuis, Cornea Service, Rotterdam Eye Hospital, Schiedamsevest 180, 3011 BH Rotterdam, the Netherlands.
  • Accepted 28 January 1997

Abstract

AIM To test the efficacy and safety of recombinant human epidermal growth factor (hEGF) on corneal re-epithelialisation following penetrating keratoplasty.

METHODS A prospective, randomised, placebo controlled study was carried out in which patients were matched for diagnosis and received either hEGF ophthalmic solution (30 μg/ml or 100 μg/ml) or placebo in a double masked fashion. Matched pairs of patients received donor corneas from the same donor and were operated by the same surgeon on the same day. At the end of surgery all donor epithelium was removed mechanically. Patients were examined twice daily and fluorescein stained photographs were taken until the epithelium had closed. The area of the defect was measured by planimetry of the fluorescein stained defect on the photographs.

RESULTS There were no significant differences in re-epithelialisation of the donor cornea between the placebo group and the group treated with 30 μg/ml hEGF. Time until complete closure was slightly longer with 100 μg/ml hEGF compared with 30 μg/ml hEGF and with placebo. Mean healing rate of the epithelial defect with 100 μg/ml hEGF was significantly slower than in the other groups.

CONCLUSION No significant acceleration of corneal re-epithelialisation was demonstrated with the use of recombinant hEGF after penetrating keratoplasty in humans.

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