Donor corneo-scleral rim cultures after organ culture
- Banque de Cornées Saint Antoine, ETS de l’APHP, Paris, France
- Vincent M Borderie, Banque de Cornées Saint Antoine, Service d’Ophtalmologie, Hôpital Saint Antoine, 184 rue du Fg St-Antoine, 75571 Paris Cedex 12, France.
- Accepted 12 February 1997
Editor,—Organ culture is now the most common corneal preservation technique used in European countries. We evaluated sterility of organ cultured donor corneas at the time of surgery.
REPORT
From December 1992 to March 1996, 396 consecutive corneas were organ cultured at our institution. Of these 396 corneas, 270 (68%) were grafted and 126 (32%) were discarded. Eyes were obtained by enucleation within 48 hours of death. At the eye bank, eyes were rinsed for 30 seconds with sterile water then immersed in a 1% povidone-iodine solution for 2 minutes.1 2 Eyes were then immersed in a 1% thiosulphate solution for 30 seconds and rinsed with sterile saline. Corneas were organ cultured in Inosol medium (Opsia, Toulouse, France) at +31°C for 2 to …
