Table 2
Primer and probe sequences used in nested polymerase chain reaction (modified from Nawa et al10)
| Sequence 5′ - 3′ | Location | Product length | |
|---|---|---|---|
| Outer | ACCGAAAACGGTTGAACCGAAAACGGT AATAATGTCTATATTCACTAATT | — | 307 bp |
| Inner: | |||
| HPV 16 | ATGTTTCAGGACCCACAGGA | 104–123 | 124 bp |
| CCTCACGTCGCAGTAACTGT | 208–227 | ||
| HPV 18 | ATGGCGCGCTTTGAGGATCC | 106–125 | |
| GCATGCGGTATACTGTCTCT | 274–293 | 188 bp | |
| Probe: | |||
| HPV 16 | CACAGTTATGCACAGAGCTGCAAACAAC TATACATGAT |
141–180 | — |
| HPV 18 | GAACTGAACACTTCACTGCAAGACATAG AAATAACCTG |
151–190 |
-
↵Consensus primers for E60RF of HPV 16 and 18.
