In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice
- aDepartment of Medical Microbiology, University of Wales college of Medicine, Cardiff CF4 4XW, bDepartment of Ophthalmology, Medical Sciences Building, University Walk, Bristol BS8 1TD
- Mr A Sharma, Cardiff Eye Unit, University Hospital of Wales, Cardiff, South Glamorgan CF4 4XW.
- Accepted 28 May 1997
Abstract
BACKGROUND/AIMS Herpes simplex virus keratitis (HSK) is the most common cause of corneal blindness in the Western world. Delay in the treatment of HSK can lead to a more significant corneal scar and topical steroid treatment in unsuspected active HSK can lead to corneal melting. Current culture techniques for herpes simplex virus (HSV) take several days and commercially available HSV laboratory based diagnostic techniques such as Herpchek vary in sensitivity. This study was conducted to assess the viability of a new, quicker, and simpler method to diagnose HSK.
METHODS Direct immunofluorescence was used in vivo in a masked study to diagnose HSK in mice using a standard slit lamp with cobalt blue illumination. Murine monoclonal fluorescently labelled antibody was applied to the cornea for 10 or 20 minutes and then washed off with phosphate buffered solution. Mice with HSK were stained with either fluorescently labelled monoclonal antibody against HSV or fluorescently labelled monoclonal antibody against cytomegalovirus. Mice with corneal abrasions of non-viral origin were given fluorescently labelled monoclonal antibody against HSV.
RESULTS Fluorescence was seen only in the mice with HSK given fluorescently labelled monoclonal antibody against HSV. This observation was confirmed upon microscopic immunofluorescent imaging of the corneal epithelial sheets.
CONCLUSION In vivo immunofluorescence may be useful in the clinical diagnosis of HSK.
