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Br J Ophthalmol 82:306-311 doi:10.1136/bjo.82.3.306
  • Original Article
    • Laboratory science

Diagnosis of Fusarium keratitis in an animal model using the polymerase chain reaction

Table 1

Summary of culture and polymerase chain reaction (PCR) results

Rabbit Eye Inoculation on day 0 Day 4 Days 5–12 Days 13–17 Days 18–21 Days 22–28
Culture Scrape PCR Swab PCR Culture Scrape PCR Swab PCR Culture Scrape PCR Swab PCR Culture Scrape PCR Swab PCR Culture Scrape PCR Swab PCR
1 R Fusarium + + + +/+ + + + −/− + +
2 L Fusarium + + + + + + + + +
3 L Fusarium −x + −x −x + +/+ + + + + +
3 R Control +x
  • These eyes received 1 mg of subconjunctival triamcinolone at the time of inoculation.

  • The control cornea was injected with broth on day 0 and thenCandida on day 14.

  • + Indicates a positive result for the presence of Fusariumby culture or PCR; − indicates a negative result for the presence ofFusarium by culture or PCR; −x indicates a false negative PCR result; +x indicates a false positive PCR result.

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