Mononuclear cell infiltration and eosinophil granule proteins in the conjunctiva of cicatricial pemphigoid
| Antibodies | Epithelium | Subepithelium | |||||
|---|---|---|---|---|---|---|---|
| Chronic | Subacute | Chronic | Subacute | ||||
| Mononuclear cell infitrate: | |||||||
| CD45 | 27.2 (15.8) | 39.6 (13.7) | 48.3 (15.4) | 62.9 (14.3) | |||
| CD3 | 9.7 (6.5) | 15.6 (4.5) | 20.3 (3.7) | 37.2 (5.6)** | |||
| HLA-DR | 34.1 (9.3) | 42.2 (14.6) | 39.3 (6.2) | 76.8 (11.9)** | |||
| Human eosinophil granule proteins: | |||||||
| EG1 | 0.01 (0.01) | 1.44 (0.44)* | 0.13 (0.08) | 1.75 (0.4)** | |||
| EG2 | 0.14 (0.06) | 0.88 (0.2)* | 0.39 (1.07) | 1.5 (0.19)** | |||
| BMK-13 | 0.08 (0.08) | 0.88 (0.38)* | 0.17 (0.1) | 2.25 (0.48)** | |||
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↵Cell numbers/high power field (450×); mean (SEM).
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↵Cellular and extracellular staining within high power field (× 450): 0 = no staining; 1+ = few foci containing light amounts of staining; 2+ = scattered foci with intense staining or larger area with light staining; 3+ = intensive staining throughout large areas.*p<0.05/**p<0.005 by Mann–Whitney U test comparing subacute and chronic disease. EG1=antibody directed against stored and secreted eosinophil cationic protein; EG2=antibody directed against secreted eosinophil cationic protein or eosinophil derived neurotoxin; BMK-13 = antibody directed against major basic protein.
