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Role of the endothelium in modulating functional responses of isolated bovine anterior ciliary arteries to vasoconstrictor agonists
  1. Christine H Buckleya,b,
  2. Patrick W F Hadokeb,
  3. Colm J O’Briena
  1. aDepartment of Ophthalmology, Royal Infirmary of Edinburgh, Edinburgh, bDepartment of Medicine, Royal Infirmary of Edinburgh, Edinburgh
  1. Dr C H Buckley, Department of Medicine, Royal Infirmary of Edinburgh, Lauriston Place, Edinburgh EH3 9YW.

Abstract

BACKGROUND/AIMS Endothelium dependent vasodilatation is an important regulator of blood flow to the eye but its role has not been investigated in vessels supplying the ciliary body. This study assessed the role of the endothelium in modulating vasoconstrictor responses of the intraocular bovine anterior ciliary artery.

METHODS Bovine anterior ciliary arteries (n=33) were mounted in a myograph, containing physiological salt solution at 37°C, for isometric force measurement. Cumulative concentration-response curves were obtained to the constrictor agonists 5-hydroxytryptamine (5-HT), noradrenaline, phenylephrine, prostaglandin F, endothelin-1, and KCl in both endothelium intact and denuded arteries.

RESULTS All vasoconstrictors produced sustained contractile responses which were unaffected by the removal of the endothelium. Responses to 5-HT were also unaffected by inhibition of nitric oxide synthase.

CONCLUSION These results indicate that neither agonist stimulated nor basal release of nitric oxide from the endothelium modulates responses to vasoconstrictor agonists in the isolated bovine anterior ciliary artery when measured in a no flow isometric system.

  • anterior ciliary artery
  • constrictor agonists
  • endothelium
  • nitric oxide

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Basal and/or agonist induced release of endothelium derived nitric oxide (NO) is a significant factor in the regulation of ocular blood flow.1-3 Consequently endothelial cell dysfunction in ocular vessels may account for the ophthalmic complications of diseases associated with dysfunction of the endothelium in the systemic vasculature (for example, diabetes, hypertension).4Similar alterations may also occur in some patients with glaucoma who have ocular or generalised vasospastic sequelae.5

The role of endothelium derived mediators in the control of vascular function in the eye varies in different arteries.2 Their contribution to tone in vessels supplying the ciliary body has not been investigated, although high levels of constitutive NO synthase (eNOS) are located in the human outflow pathway and ciliary muscle.6 This may be relevant to the development of glaucoma as patients with this condition, which is associated with an increase in both outflow resistance and intraocular pressure (IOP), have a reduced distribution of NO containing cells in the outflow pathway.7 Furthermore, nitrovasodilators, which mimic the actions of NO, reduce IOP through a mechanism involving alterations in the resistance to aqueous humour outflow.8

The limited data available on normal physiological control of ocular blood vessels make it difficult to describe the exact vascular alterations which occur during disease progression. The development of the small vessel myograph by Mulvany and Halpern9 has provided a technique which can be used to investigate vascular function in vitro using vessels with an internal diameter of as little as 100 μm. The aim of the present study was to investigate the role of the endothelium in mediating vasoconstriction in the intraocular bovine anterior ciliary artery.

Materials and methods

TISSUE PREPARATION

Bovine eyes were obtained from the abattoir and transported in cold physiological salt solution (PSS) of the following composition (mM): NaCl 119, KCl 4.7, MgSO4 1.17, KH2PO4 1.18, glucose 5.5, K2EDTA 0.026, NaHCO3 25, CaCl2 2.5. The posterior segment of the eye together with the vitreous was removed, intraocular anterior ciliary arteries were dissected from beneath the choroid and transferred to the chamber of a wire myograph (Model 400A, JP Trading, Aarhus, Denmark).

Arterial rings, approximately 2 mm in length, were mounted on two 40 μm intraluminal wires for measurement of isometric force development. The vessels were allowed to equilibrate in PSS at 37°C and gassed with 95% oxygen, 5% carbon dioxide for 30 minutes before undergoing normalisation using standard methodology.10 Briefly, this involved stepwise stretching of the vessel and application of the LaPlace relation to determine the internal circumference (L100) of a vessel when relaxed and under an effective transmural pressure of 100 mm Hg (13.3 kPa). The vessel was then set at an internal circumference of 0.9 L100 at which many small arteries,10 including bovine11 and canine12 ocular arteries develop maximum or near maximum active tension. This setting has been used previously in functional investigations of bovine anterior ciliary arteries.13 The vessels were then left to equilibrate under their normalised tension for 30 minutes.

The functional integrity of the endothelium was assessed in each artery by the addition of bradykinin (BK; 10−6 M) following contraction with 5-hydroxytryptamine (5-HT; 3 × 10−7M). The endothelium was considered intact if BK evoked a relaxation of greater than 60% of the 5-HT induced tone. In some arteries the endothelium was removed by gently rubbing the lumen of the vessel with a human hair. Histological studies have confirmed that this method successfully removes the endothelium14and denudation was confirmed in the present study by the failure of the vessel to relax in response to BK (10−6 M).

INFLUENCE OF THE ENDOTHELIUM ON VASOCONSTRICTOR RESPONSES

The role played by the endothelium in modulating functional responses of bovine anterior ciliary arteries to vasoconstrictors was investigated using a variety of agonists. Cumulative concentration-response curves were obtained to the vasoconstrictors 5-HT (10−9–3 × 10−5 M), noradrenaline (NA; 10−9–3 × 10−5 M), phenylephrine (PE; 10−8–3 × 10−3 M), prostaglandin (PG) F (10−8–3 × 10−5M), endothelin-1 (ET-1; 10−11–3 × 10−7M), and KCl (10–125 mM) in both endothelium intact and denuded vessels. Following each concentration-response curve the vessels were washed thoroughly with PSS and allowed to relax fully for at least 20 minutes before the next drug was tested.

The role of endothelium derived NO in the modulation of 5-HT induced contraction was assessed by constructing concentration-response curves in the absence of, and following incubation of arteries for 45 minutes with, the NO synthase inhibitor NG-nitro-l-arginine (L-NNA; 10−4M).

STATISTICAL ANALYSIS

Contractile responses of the vessels were expressed as active wall tension (mN/mm) and relaxation responses as a percentage of the induced contraction. For each concentration-response curve the molar concentration required to produce 50% of the maximum contraction (EC50) was calculated by fitting the data to the Hill equation using the curve fitting program fig p (Biosoft, UK). The sensitivity of the vessels to each constrictor agonist was expressed as the negative log EC50 (pD2). The results are given as the mean (SEM) for n separate experiments; only one artery was used from each animal and so n also refers to the number of animals used.

Maximum contraction and EC50 values were compared using Student’s unpaired t test and considered significantly different when p⩽0.05.

Results

Preliminary experiments demonstrated that vessels remained viable if stored in PSS at 4°C for 24 hours, with both vasoconstrictor and vasodilator function unaltered (data not shown). This extended the period during which experiments could be performed. The mean normalised lumen diameter of vessels used in the present study was 218 (6) μm (n=33). Vessels which had not had the endothelium removed relaxed by 79.8% (4.5%) (n=11) in response to BK, whereas in denuded arteries the relaxation was reduced to 5.1% (3.3%) (n=8) (Fig1).

Figure 1

Responses to bradykinin (10−6 M) in endothelium intact bovine anterior ciliary arteries precontracted with 5-HT (3 × 10−7 M) in the absence (open column; n=11) and presence (hatched column; n=8) of L-NNA (10−4 M), and in endothelium denuded vessels (solid column; n=8). Each column represents a mean value with vertical bars indicating SEM. *p<0.01 compared with endothelium intact vessels without L-NNA.

In quiescent arteries with an intact endothelium all six agonists tested, 5-HT, NA, PE, PGF, ET-1, and KCl, produced concentration dependent contractions (Table 1). Concentration-response curves to 5-HT in both endothelium intact and denuded arteries are shown in Figure 2. A similar pattern of response was seen for each of the other five agonists, except for PE and PGF, which failed to reach a stable maximum contraction in the concentration ranges used. Consequently, the pD2 values calculated for PE and PGF (Table 1) are only approximate. Removal of the endothelium did not alter the sensitivity (pD2) to any of the agonists (Table 1). In all cases, the maximum contractile response was slightly, but not significantly, reduced in endothelium denuded arteries compared with intact vessels (Table 1).

Table 1

Effect of endothelium removal on constrictor responses of bovine anterior ciliary arteries

Figure 2

Cumulative concentration-response curves to 5-HT in endothelium intact (▴; n=12) and denuded (□; n=10) bovine anterior ciliary arteries. All values are means (SEM).

Addition of 10−4 M L-NNA (a concentration shown to inhibit BK mediated relaxation in the anterior ciliary artery (Fig 1)) did not cause an increase of tone in any of the isolated arteries (data not shown). Furthermore, incubation with L-NNA (10−4 M) did not alter the sensitivity or the magnitude of the contraction induced by 5-HT (Fig 3).

Figure 3

Cumulative concentration-response curves to 5-HT in endothelium intact bovine anterior ciliary arteries incubated for 45 minutes with the nitric oxide synthase inhibitor L-NNA (10−4 M;▴ ; n=4) and in vehicle treated controls (□; n=4). All values are means (SEM).

Discussion

This investigation used small vessel myography to investigate the role of the endothelium in modulating agonist induced vasoconstriction in the bovine anterior ciliary artery. The results have demonstrated that this artery contracts in response to a variety of agonists and indicate that neither basal nor agonist induced release of NO from the endothelium modulates this contraction.

The vascular endothelium makes a major contribution to local vascular tone and blood flow through the release of both vasodilator (NO and prostacyclin) and vasoconstrictor (ET-1 and cyclo-oxygenase products) agents.15 Any disturbance to the balance between constrictor and dilator compounds released by the endothelium may result in vasospasm and alterations in blood flow.4Furthermore, many agonists which contract healthy arteries may also contribute to vasodilatation by the release of NO from the endothelium. In arteries with dysfunctional endothelium the vasodilator responses are lost, exacerbating the vasoconstrictor response.16Therefore, endothelial cell dysfunction may alter both the basal vascular tone and the response of the vessel to circulating agonists and pharmacological agents and, consequently, is an important factor in both the development and treatment of disorders of the eye.

Functional responses of ocular vessels are heterogeneous, depending upon the anatomical origin of the vessel, its location within or outside the orbit, and also upon the part of the vessel selected (reviewed by Buckley et al17). In the present investigation, vasoconstrictor agonists were used to provide information on the role of the endothelium in modulating the action of agonists derived from sympathetic nerves, aggregating platelets and damaged endothelial cells.18 KCl, a receptor independent vasoconstrictor, was included as a control and PGF was also included as many investigations have used this to precontract vessels for subsequent investigation of vasodilator activity.13 19 These agonists all produced sustained, concentration dependent responses that were not altered significantly by removal of the endothelium. The slight, but not significant, reduction in the maximum contraction following removal of the endothelium was probably due to slight damage to the vascular smooth muscle cells which is a recognised problem encountered when denuding small arteries. This was supported by the demonstration that inhibition of NO synthase using L-NNA did not result in a reduction of the magnitude of 5-HT induced contraction. Further experiments are required to investigate the effect of NO synthase inhibition on the responses to other constrictor agonists and also to determine the potential role of other vasoactive mediators such as PGI2 in regulating blood flow in this artery. These results suggest that basal release of NO, or any other vasoactive agent, is not a significant factor in bovine anterior ciliary arteries in an isometric system. This observation is supported by the absence of an increase in basal tone following the addition of thel-arginine analogue (L-NNA). In contrast, in vivo experiments using NO synthase inhibitors have demonstrated a significant reduction in blood flow to the anterior uvea in both rabbits and dogs indicating that basal NO release is involved in the regulation of uveal blood flow under resting conditions.20 21 Whether this difference reflects the different species used or the size of the vessels or is due to the different methodological approaches used requires further investigation. Investigations using porcine isolated ocular arteries have suggested that the influence of the endothelium and endothelium derived NO on contractile responses is dependent upon the size and location of a particular vessel.1 2 Basal release of NO is more important in larger arteries, modulating vasoconstriction in porcine ophthalmic, but not ciliary, arteries.2 The results reported for the porcine ciliary artery2 are consistent with those obtained in the present study, whether the influence of the endothelium or endothelium derived NO on contractile response depends on vessel size in the bovine ophthalmic vascular bed, or more importantly in the human, remains to be determined.

The use of NA and PE demonstrated that α adrenoceptors were present in the anterior ciliary artery. Sensitivity to the specific α1 adrenoceptor agonist, PE, was 100-fold lower than that to NA, suggesting that the latter may contract this vessel by stimulation of more than one receptor subtype. Indeed, bovine intraocular long posterior ciliary arteries have been shown to contract in response to both selective α1 and α2adrenoceptor agonists.13 In contrast, α2adrenoceptor agonists do not cause contraction in canine or human ciliary arteries with the contraction to NA in these vessels mediated solely by α1 adrenoceptors.22-25 NA can also relax some arteries by stimulating β adrenoceptors or by α2 adrenoceptor mediated release of NO from the endothelium.26 As NA mediated vasoconstriction was unaffected by removal of the endothelium in the present study, α2 adrenoceptor mediated release of NO appears not to play a role in regulating tone in the bovine anterior ciliary artery.

The inhibitory action of NO on platelet aggregation may become impaired in patients with endothelial cell dysfunction, allowing aggregating platelets to adhere to the vascular wall (releasing vasoactive agonists, including 5-HT), with consequent vasospasm.27The 5-HT induced vasoconstriction in the present investigation is most likely to be mediated by 5-HT2 receptors on the vascular smooth muscle cells as demonstrated in porcine ophthalmic and ciliary arteries.2 5-HT can also cause vessels to dilate by acting on 5-HT1 receptors on the endothelium to release NO28 but this does not appear to be significant in the bovine anterior ciliary artery as neither removal of the endothelium nor incubation with L-NNA increased its sensitivity to this agonist.

The vasoconstrictor ET-1 is released by damaged or hypoxic endothelium and increased plasma concentrations of ET-1 are associated with several vascular diseases (reviewed by Haynes and Webb29) including some types of glaucoma.30 ET-1 induces vasoconstriction by stimulation of ETA (and to a lesser extent ETB receptors) on the vascular smooth muscle but can also induce dilatation by stimulating ETB receptors on the endothelial cells.18 ETB mediated release of endothelium derived vasodilators does not appear to modulate ET-1 induced contraction in isolated bovine anterior ciliary arteries as removal of the endothelium did not increase the vascular sensitivity to this agonist.

In conclusion, the results of the present study show that the endothelium does not modulate responses of isolated bovine anterior ciliary arteries to a variety of constrictor agonists. Furthermore, there does not appear to be a significant basal release of NO by this artery when studied in an isometric system in vitro. This suggests that endothelial cell dysfunction associated with cardiovascular diseases is unlikely to significantly reduce blood flow to the ciliary body.

Acknowledgments

This work was supported in part by grants from the Royal College of Surgeons of Edinburgh and the Royal School for the Blind, Edinburgh, UK.

References

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