Table 1
Staining and spectral features of the different agents used in fluorescent leucocyte angiography
| Fluorescent agent | Molecular weight | Staining features | Absorption wavelength (nm) | Emission wavelength (nm) |
|---|---|---|---|---|
| Acridine orange | 302 | Binds to nucleic acids to produce fluorescent chromophore | 500 | 526 |
| Carboxyfluorescein diacetate (CFDA) | 460 | Converted by intracellular esterases to a fluorescent derivative | 492 | 517 |
| Calcein-am | 995 | Converted by intracellular esterases to a fluorescent derivative | 494 | 517 |
| Fluorescein isothiocyanate (FITC) | 389 | Binds to proteins | 494 | 519 |
| Sodium fluorescein | 376 | Binds to proteins | 485 | 530 |
| Indocyanine green | 775 | Binds to proteins | 805 | 835 |
