Table 1
Number of vitreous and SRF samples that were positive for presence of mRNA for the different cytokines tested
| HPRT | IL-6 | IL1-β | TNFα | IL-8 | |
|---|---|---|---|---|---|
| Sample | |||||
| Vitreous (12) | (12) 100% | (10) 83% | (6) 50% | (6) 50% | (9) 75% |
| SRF (8) | (8) 100% | (6) 75% | (6) 75% | (4) 50% | (6) 75% |
| Total (20) all samples | (20) 100% | (16) 80% | (12) 60% | (10) 50% | (15) 75% |
| Controls | |||||
| MH (10) | (5) 50% | (2) 20% | (1) 10% | (0) 0% | (1) 10% |
| RD (6) | (6) 100% | (2) 33% | (0) 0% | (1) 16.5% | (2) 33% |
| Total (16) control | (11) 68.75% | (4) 25% | (1) 6.25% | (1) 6.255 | (3) 18.75% |
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SRF = subretinal fluid, MH = vitreous from macular hole patients, RD = vitreous from retinal detachment patients. Primers used: HPRT 5′GACCAGTCAACAGGGGACAT/3′CGACCTTGACCATCTTTGGA (160 base pairs), IL-6 5′AGGTTGTTTTCT GCCAGTGC/3′CACACAGACAGCCACTCACC (145 base pairs), IL-1β 5′AGCCATGGCAGAAGTACCTG/3′CATCTG TTTAGGGCCATCAG (100 base pairs), TNFα 5′CACCACGCTCTTCTGCCT/3′TCTCAGCTCCACGCCATT (225 base pairs), IL-8 5′AAGAAACCACCGGAAGGAAC/3′TGTGGTCCACTCTCAATCACTC (218 base pairs).
