Absolute cell numbers (×104)/animal (two retinas)
| Cell populations/days post immunisation | Controls | Tolerised | |||||
|---|---|---|---|---|---|---|---|
| 7 | 14 | 7 | 14 | ||||
| CD4+αβTCR+ | 1.62 | 109 (130) | 1.44 | 69.7 (58.3) | |||
| CD8+αβTCR+ | 1.17 | 42 (47.1) | 1.86 | 28.7 (16.8) | |||
| ED7+CD4+ | 24.3 | 67.1 (54.8) | 12.3 | 24.6 (37.3) | |||
| ED7+MHC class II+ | nd | 109.8 | nd | 98.4 | |||
| OX22+CD4+ | nd | 51 | nd | 41 | |||
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↵Figures are a representative experiment and calculated from the mean recovery from two animals in each group (four retinas). (Cell numbers for a second experiment in parenthesis.)
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↵Cells were backgated from CD45(OX1)+ positive cells (see Methods) and only ED7high cells were included in analysis of macrophage infiltrate thereby excluding resident microglia (ED7low expressing; see Dick et al 22) which did not change in number between groups in any of the experiments.
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↵See Figure 3 and legend.
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↵Athough MHC class II expression was increased on majority of CD45(OX1)+ cells greater than negative control, increased numbers of ED7+ cells expressed MHC class II in control retinas (see Fig 3).
