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Analysis of glycosaminoglycans in rabbit cornea after excimer laser keratectomy
  1. Takuji Katoa,
  2. Kiyoo Nakayasua,
  3. Kazuyuki Ikegamib,
  4. Takeo Obarab,
  5. Toshiji Kanayamab,
  6. Atsushi Kanaia
  1. aDepartment of Ophthalmology, Juntendo University, Tokyo, Japan, bShiseido Pharmaceutical Research Laboratories, Yokohama, Japan
  1. Takuji Kato, MD, Department of Ophthalmology, Juntendo University, 3-1-3 Hongo, Bunkyo-ku, Tokyo 113, Japan.


BACKGROUND/AIMS The biochemical basis for the development of subepithelial opacity of the cornea after excimer laser keratectomy has yet to be fully defined. The aim of this study was to evaluate the alterations of glycosaminoglycans (GAGs) after excimer laser keratectomy.

METHODS Rabbit corneas were harvested on days 5, 10, 20, and 30 after excimer laser photoablation. The amount of main disaccharide units was determined by high performance liquid chromatography (HPLC). In addition, immunohistochemical studies were performed on corneal sections 20 days after the ablation.

RESULTS The concentrations of ΔDi-0S at 5 and 10 days were significantly lower than before the ablation. ΔDi-6S showed a significant increase 5 days after the ablation but ΔDi-4S did not show any significant change. There was a significant increase in ΔDi-HA at 20 and 30 days after ablation. In immunohistochemistry, the positive staining for ΔDi-6S and hyaluronic acid was observed in the subepithelial region. These immunohistochemical results were well correlated with the HPLC findings.

CONCLUSIONS The increase in chondroitin-6 sulphate and hyaluronic acid may be related to corneal subepithelial opacity after excimer laser keratectomy.

  • excimer laser
  • glycosaminoglycans
  • high performance liquid chromatography
  • immunohistochemistry
  • rabbits

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