Effect of the cytostatic agent idarubicin on fibroblasts of the human Tenon’s capsule compared with mitomycin C
- aDepartment of Ophthalmology, Otto-von-Guericke-Universität Magdeburg, Germany, bInstitute of Biochemistry, Otto-von-Guericke-Universität Magdeburg, Germany
- W Behrens-Baumann, MD, Department of Ophthalmology, Otto-von-Guericke-Universität, Leipziger Strasse 44, D 39120 Magdeburg, Germany.
- Accepted 25 March 1999
Abstract
BACKGROUND/AIMS To investigate the in vitro effect of a short time exposure to the anthracycline idarubicin on proliferation, protein synthesis, and motility of human Tenon’s capsule fibroblasts in comparison with the antitumour antibiotic mitomycin C.
METHODS After determination of effective concentrations of idarubicin, fibroblasts of the human Tenon’s capsule were exposed to idarubicin or mitomycin C at concentrations ranging from 0.1 μg/ml to 1 μg/ml or from 2.5 μg/ml to 250 μg/ml, respectively, for 0.5, 2, or 5 minutes and cultured for 60 days. Cell death by apoptosis caused by idarubicin treatment was confirmed by Hoechst 33258 staining. Further proliferation was explored by cell counting and by3H-thymidine uptake. Protein synthesis was measured by3H-proline uptake and motility was assessed by agarose droplet motility assay.
RESULTS Idarubicin is able to exert toxicity and to induce apoptosis during a short time exposure of 0.5 minutes at concentrations of 0.3–1 μg/ml resulting in a significant reduction in cell number compared with the control after 60 days. For mitomycin C, higher concentrations and longer expositions were necessary. Even after treatment with 1 μg/ml idarubicin or 250 μg/ml mitomycin C a few cells were able to incorporate 3H-thymidine. 3H-proline uptake up to 10 days after exposure to 0.3 μg/ml idarubicin was found not to be decreased. Cell motility was reduced after treatment with 1 μg/ml idarubicin for 5 minutes or with 250 μg/ml mitomycin C for 2 or 5 minutes. For low mitomycin C concentrations, an increase in motility was found during the first 10 days.
CONCLUSION Idarubicin reduces proliferation of human Tenons’s capsule fibroblasts after incubation for 0.5 minutes at concentrations as low as 0.3–1 μg/ml. In comparison, mitomycin C requires longer exposure times and higher doses for equal results. Therefore, idarubicin may be useful in the prevention of glaucoma filtering surgery failure.
