Table 1
Monoclonal antibodies used to characterise PEC
| MoAbs | Source | DF | IR | Specificity |
|---|---|---|---|---|
| AE1 | ICN | 1:50 | + | Acidic keratins (type I) |
| AE3 | ICN | 1:50 | + | Basic keratins (type II) |
| AE5 | ICN | 1:50 | + | 64 kDa keratin (corneal type differentiation) |
| AE8 | ICN | 1:50 | − | 51 kDa keratin (non-keratinising epithelium) |
| AE1/AE3 | Dako | 1:50 | + | Pan-cytokeratin (acidic and basic keratins) |
| MUC-1 | Serotec | 1:50 | + | Mucin |
| ESA | Zymed | 1:50 | + | 40 kDa epithelial antigen |
| VM | Dako | 1:20 | + | 57 kDa intermediate filament protein |
| CD1 | Dako | 1:50 | − | Langerhans cells |
| CD68 | Dako | 1:50 | − | Macrophages |
| vWF | Dako | 1:50 | − | Endothelial cells |
| Mouse IgG1 | Dako | 1:50 | − | Non-specific |
| C-11FITC | Sigma | 1:20 | + | Pan-cytokeratin (acidic and basic keratins) |
| IgG1 FITC/IgG2α PE | BD | 1:20 | − | Not specific |
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BD = Becton Dickinson; DF = dilution factor; ESA = epithelial specific antigen; IR = immunoreactivity; MoAbs = monoclonal antibodies; VM = vimentin; vWF = von Willebrand factor; (+) positive and (−) negative specific immunoreactivity in cultured PEC. Becton Dickinson, Dako, ICN, and Sigma (Sydney, Australia); Zymed, (San Francisco, CA, USA); Serotec, (Oxford, UK).
