rss
Br J Ophthalmol 2000;84:98-102 doi:10.1136/bjo.84.1.98
  • Original Article
    • Laboratory science

Effects of proton beam irradiation on uveal melanomas: a comparative study of Ki-67 expression in irradiated versus non-irradiated melanomas

  1. Christophe Chiqueta,b,
  2. Jean-Daniel Grangea,
  3. Louis Ayzacc,
  4. Pierre Chauveld,
  5. Louis-Marc Patricotb,
  6. Mojgan Devouassoux-Shisheboranb
  1. aDepartment of Ophthalmology, Croix-Rousse Hospital, University of Lyons, France, bDepartment of Pathology, cDepartment of Biostatistics, Lyon-Sud Hospital, University of Lyons, France, dBiomedical Cyclotron, Nice, France
  1. Jean-Daniel Grange, MD, Department of Ophthalmology, Croix-Rousse Hospital, 93, grande Rue de la Croix-Rousse, 69317 Lyon cedex 04, France
  • Accepted 18 August 1999

Abstract

AIMS To assess the cellular proliferation using the monoclonal antibody Ki-67, in paraffin embedded uveal melanomas irradiated by proton beam, as well as in non-irradiated uveal melanomas.

METHODS 30 enucleated eyes were included for histopathological study and Ki-67 immunostaining. Patients were enucleated between 1991 and 1996 for uveal melanoma, 14 after proton beam irradiation and 16 without treatment (control group). The mean follow up period was 2.5 years after diagnosis and 1 year after enucleation.

RESULTS A significant relation was found between Ki-67 score and mitotic index (r = 0.56, p = 0.001), histological largest tumour diameter (r = 0.38, p = 0.03), fibrosis (r = −0.35, p = 0.05), absence of tumoral pigmentation (p = 0.05), and presence of vascular thrombosis (p = 0.03). The Ki-67 score was significantly higher in the non-irradiated group (p = 0.01) and in the group of patients whose cause of enucleation was tumoral evolution (p = 0.005) compared with the group of patients enucleated after neovascular glaucoma. The Ki-67 score was very high in a case of orbital recurrence of uveal melanoma and metastatic death. 70% of metastasised tumours showed a Ki-67 score higher than the median value.

CONCLUSION Ki-67 labelling is a reliable method of estimating the proliferative activity in uveal melanomas after proton beam irradiation. The Ki-67 score is significantly correlated with prognostic variables (mitotic index and histological largest tumour diameter), and with radiation effects after proton beam irradiation.

Footnotes

    This Article

    1. Abstract
    2. Full text
    3. PDF

    Responses

    1. Submit a response
    2. No responses published

    Register for free content

    The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.

    Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.