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Br J Ophthalmol 84:1238-1243 doi:10.1136/bjo.84.11.1238
  • Original Article
    • Clinical science

Detection of herpes simplex virus type 1, 2 and varicella zoster virus DNA in recipient corneal buttons

Table 3

HSV-1 antibodies and HSV-1 DNA in aqueous humour at the time of perforating keratoplasty

Diagnosis at PKP (n) PCR HSV-1 in corneal button PCR HSV-1 in aqueous humour Intraocular anti HSV antibody production
positive: negative: positive: negative:
HSK, primary graft (20) positive:  8 0  6 4  4
negative: 12 0 12 1 11
Allograft failure with initial HSK diagnosis (11) positive:  2 0  2 0  2
negative:  9 0  7 4  2
Allograft failure with initial non-HSK diagnosis (17) positive:  8 0  6 2  2
negative:  9 0  8 1  4
Ulcers, non-specified (9) positive:  3 0  3 0  2
negative:  6 0  6 0  3
Other diagnosis, non-HSK (52) positive:  2 0  1 0  1
negative: 50 0  3 0  3
  • In a great number of cases, the amount of AH was insufficient to detect antibody production and perform PCR;

  • the ELISA did not discriminate between HSV-1 and HSV-2;

  • in 1 case the GWc for VZV was also positive.

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