BACKGROUND/AIMS Accumulating evidence indicates that telomerase activity is repressed in normal human somatic cells but reactivated in cancers and immortal cells, suggesting that activation of telomerase activity has a role in carcinogenesis and immortalisation. To date, telomerase in uveal melanoma and, whether, it may have a role in the development or progression of these tumours has not been described. The expression patterns and the activity of telomerase were investigated in 14 uveal melanoma and these results were correlated with histological and immunohistological features of these tumours.
METHODS A modified PCR based telomeric repeat amplification protocol (TRAP) assay was used to demonstrate telomerase activity in 14 uveal melanomas. In addition, in situ hybridisation was used to demonstrate the expression pattern of the telomerase RNA component (hTR) at the single cell level in eight of these globes.
RESULTS The TRAP assay revealed moderate telomerase activity in all uveal melanomas examined. In situ hybridisation visualised a moderate to high upregulation of hTR in the melanoma cells but not in the admixed reactive cells. There was no correlation among tumour location, cell type, or growth fraction and the amount of telomerase activity. In addition, the cells of the germinative zone of the lens demonstrated a strong hTR expression.
CONCLUSION Telomerase activity is upregulated in uveal melanomas. The expression of hTR was located to the tumour cells and not the reactive tumour infiltrating cells. Strong telomerase expression was also demonstrated in cells of the germinative zone of the lens.
- uveal melanoma
- telomerase activity
- monosomy 3
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Telomeres are chromosomal end regions made of several thousand copies of a repeating nucleotide sequence (in human 5′-TTAGGG-3′)1 and are important in stabilising the chromosome during replication. In human cells, DNA polymerase is not able to completely replicate the linear DNA of the telomeres,2 3 resulting in the loss of 50–200 nucleotides from the telomere with each cell division.4 It has been proposed that this progressive shortening of telomeres in normal somatic cells acts as an internal clock by which the cell counts replication events. Cell senescence acts as an anticancer mechanism and is thought to occur when the telomere length is critically shortened.5
Telomerase, a ribonucleoprotein polymerase, is capable of synthesising telomeric DNA onto the ends of chromosomes, compensating the telomeric sequences usually lost with cell divisions.6 According to the telomere hypothesis, only those cells which acquire telomerase activity retain the chromosomal stability, altering the cell's biological clock and producing an immortal phenotype.1 7This hypothesis has been supported by data demonstrating telomerase activity in a wide variety of malignant cells and tissues,8-15 but not in most normal tissues. Telomerase activity is, however, also described in physiologically proliferating cells such as lymphocytes and basal epithelial cells.16-20 Therefore, telomerase activity measured in whole tissue lysates using, for example, a polymerase chain reaction (PCR) based telomeric repeat amplification protocol (TRAP), is not sufficient to confirm the malignant nature of a mixed lesion.
Telomerase is composed of ribonucleic acid (RNA) and protein components. The human telomerase RNA component (hTR) has been recently cloned and shown to be critical for telomerase activity.21Telomerase RNA expression levels determined by northern blot analysis or reverse transcriptase (RT) PCR suffer from similar drawbacks as the TRAP assay since a cellular localisation of the obtained signals is not possible.22 23 Applying in situ hybridisation for hTR, it was possible to demonstrate high upregulation of hTR in dysplastic cells whereas reactive proliferating cells exhibit only low to moderate signals.10 24-27
Uveal melanoma is the most frequent primary intraocular tumour in white adults, with an incidence of 0.7 per 100 000.28 The pathogenesis of uveal melanoma is unclear and probably represents a multistep process involving the progressive and clonal accumulation of multiple genetic lesions affecting proto-oncogenes and tumour suppressor genes. Recent studies have demonstrated certain features of these tumours, which may suggest certain genetic changes in their development and progression. These include an overexpression of the cell cycle regulator proteins such as p53,29 30p16,31 and cyclin D1,32 as well as chromosomal abnormalities, in particular, monosomy 3.33-35 To date, neither the pattern of telomerase expression nor its activity has been investigated in uveal melanoma. In the present study, we investigated telomerase activity in 14 cases of uveal melanomas and confirmed that this activity is derived from the tumour cells by in situ hybridisation and not from admixed reactive cells, such as infiltrating lymphocytes and macrophages. Furthermore, the in situ hybridisation for hTR demonstrated strong signals over equatorial cells of the lens, and occasional ganglion cells of the retina.
PROCUREMENT AND PREPARATION OF SPECIMENS
Thirteen eyes were enucleated for uveal melanoma; in one patient orbital manifestation of uveal melanoma was examined. Before fixation in 4% formalin, the enucleated eyes were opened and biopsies of the tumour were removed and immediately frozen in liquid nitrogen and stored at −80°C until use. The remaining globes were fixed in formalin and embedded in paraffin for sectioning. Conventional histological stains were assessed for cell type using the modified Callender system.36
Additional slides were stained for immunohistochemical studies using a monoclonal antibody that are reactive in paraffin sections. An antigen retrieval method using a pressure cooker was performed before immunohistochemical staining.37 Conventional indirect immunohistochemistry was applied following standard protocols.38 The staining consisted of incubation with the primary monoclonal antibody MIB-1 (directed against the Ki-67 antigen, expressed in the late G1, M, G2, and S phase, but not in the early G1 and G0 phases),39-41 and it was made visible using alkaline phosphatase anti-alkaline phosphatase (APAAP). In heavily pigmented tumours, the sections were placed in hydrogen peroxide for 18 hours to remove melanin pigmentation from the tumour cells before cover slipping the slides, as previously described.42 Cells were considered positive for MIB-1 only when distinct nuclear staining was identified. The percentage of immunoreactive nuclei in the uveal melanoma was evaluated by counting at least 5 × 100 cells using the 40× objective (Olympus, BH2). The mean of these values was expressed as a percentage and reflected the “growth fraction” of the tumours.
To investigate tissue samples for the presence of telomerase activity, 15 μm thick tissue sections were produced from fresh frozen tissue blocks and controlled by histological examination of 4–7 μm sections directly above and beneath the extracted tissue proportion to confirm the presence of the expected lesion. As a positive control for the TRAP assay, the cell line used (Hela cells derived from cervical carcinoma) was cultured in RPMI medium supplemented with 10% FCS. Cell suspensions were centrifuged at 1000 g for 10 minutes and washed twice with phosphate buffered saline. Depending on their size, the sections and cell pellets were homogenised in 200–300 μl of lysis buffer (10 mM TRIS-HCl (pH 7.5), 1 mM MgCl2, 1 mM EGTA, 0,1 mM phenylmethylsuphonyl fluoride, 5 mM β-mercaptoethanol, 0.5% CHAPS (Pierce, Oud-Beijerland, Netherlands), 10% glycerol), incubated at 4°C for 30 minutes, and centrifuged at 100 000 g for 30 minutes at 4°C. The supernatants were removed and stored at −80°C until use.
Telomerase activity was determined according to Kimet al 8 using the non-radioactive GeneScan method as described previously10 11 with minor modifications using 0.1 mM spermidine (Sigma) replacing T4 protein according to Aldous and Grabill.43 Briefly, an aliquot of 6 μg, 0.6 μg, and 0.06 μg of total protein from each sample was investigated. Two TRAP assays were independently performed from each sample. Each assay was carried out including positive control (Hela cells; dilution from 6 μg, 0.6 μg, 0.06 μg, and 0.006 μg protein) and a negative control (lysis buffer added to the complete TRAP mixture).
ANALYSIS AND QUANTIFICATION OF THE TRAP ASSAY
TRAP positive samples were semiquantified employing the peak areas between 37 bp and 150 bp of the TRAP amplification products as described elsewhere.10 11 The sum of corresponding peak areas of a positive control (K562; 0.6 μg protein) obtained from the same GeneScan run was set to 100 units and the peaks of the negative control (lysis buffer) were set to 0 units. The relative telomerase activity (rTA) of a given sample X (0.6 μg protein) was calculated using the following formula:
rTAX = [((X1−nc)/(pc−nc) + (X2−nc)/(pc−nc))/2 × 100]units (X1, X2=peak areas of sample X obtained from two different TRAP assays; pc=peak area, positive control, nc=peak area, negative control).
IN SITU HYBRIDISATION
In situ hybridisation for telomerase expression was carried out as described previously.10 11 In brief, the probe for detection of the human telomerase RNA component (hTR) by in situ hybridisation was generated by RT-PCR employing primers deduced from published sequences: TEL-UP 5′-GGTGGCCATTTTT- TGTCTAAC-3′; TEL-LOW 5′-TGCATGT- GTGAGCCGAGT-3′. Total RNA from phytohaemagglutinin (PHA) activated peripheral blood lymphocytes (1 μg) was used for reverse transcription and amplification as described above. The resulting 417 bp amplificates were subcloned in pAMP1 (Gibco BRL, Gaithersburg) and sequenced (377A, Perkin-Elmer/Applied Biosystems, Weiterstadt, Germany). Hybridisation was carried out with 35S labelled run off transcript as described elsewhere.44 In brief, dewaxed and rehydrated paraffin sections (4 μm thick) were exposed to 0.2 N HCl and 0.125 mg/ml pronase (Boehringer Mannheim, Germany), followed by acetylation with 0.1 M triethanolamine pH 8.0/0.25% (v/v) acetic anhydride and dehydration through graded ethanol. Slides were hybridised to 2–4×105 cpm of labelled probes overnight at 50°C. After an exposure time between 4–12 weeks, the slides were developed and counterstained with either haemalaun or haematoxylin and eosin.
Clinical details as well as histomorphological data of the tumours examined are summarised in Table 1. The patients with uveal melanoma were 10 females and four males with an age range 31–87 years (mean 60 years). Thirteen patients had been treated with primary enucleation; in one patient an orbital exenteration was performed following localised tumour recurrence after globe enucleation. The follow up period varied between 2 and 5 years (mean 3.5 years). Two of the patients had died from their disease; four patients were alive with clinical evidence of liver metastases, and eight patients were alive without evidence of metastases. The majority of the uveal melanomas consisted of mixed epithelioid and spindle melanoma cells with a growth fraction between 5 and 20% (mean 10%).
Moderate telomerase activity could be demonstrated in all choroidal melanomas investigated by the TRAP assay ranging from 20 to 40 units (Fig 1). In situ hybridisation revealed moderate to high expression of hTR over the nuclei of melanoma cells, whereas infiltrating reactive lymphocytes and macrophages did not demonstrate any specific signal accumulation (Fig 2A and B). These results serve as a confirmation that the measured telomerase activity is derived from the malignant tumour cells only. The hTR expression was uniform and did not vary according to the location or the cell type of the tumour. Further, there was neither a correlation between the tumour telomerase activity nor hTR upregulation and the growth fraction of the tumour.
Additional findings included moderate to strong upregulation of hTR in the few equatorial cells of the lens (Fig 2C and D) as well as mild to moderate positivity in scattered ganglion cells of the retina (Fig 2E and F). This positivity in the retina was observed in both tumour adjacent and distant areas. Further, it was not associated with a retinal detachment—that is, retinal ischaemia. Cells which were negative for hTR expression in the eye included the corneal epithelial cells, the corneal endothelium, the endothelial cells lining the trabecular meshwork, the pigmented and non-pigmented layers of the ciliary epithelium, the iris, the melanocytes of the choroid in areas distant from tumour, the remaining cell layers of the retina, including the retinal pigmented epithelium, and the optic nerve.
Telomerase is linked to the immortalisation of cells and has been found in most human malignancies. We examined the telomerase expression profile in uveal melanomas and compared it with certain clinical (age, outcome) and morphological features (tumour size, location, cell type, and growth fraction). Furthermore, we performed in situ hybridisation of the whole eye to study the expression of the telomerase RNA component (hTR) at the single cell level in both tumorous and non-tumorous parts of the eye.
All uveal melanomas examined in this study exhibited telomerase activity. The application of in situ hybridisation for hTR confirmed the activity to be derived from the tumour cells and not from the admixed infiltrating lymphocytes or macrophages, frequently seen in uveal melanoma. Further, a positivity for hTR was not observed in normal choroidal melanocytes distant from tumour, indicating that a telomerase upregulation is present in uveal melanomas. This finding, one in common with all or almost all human malignancies, underlines the importance of a telomerase upregulation in tumorigenesis. The amount of telomerase activity found in uveal melanomas was moderate, with only minimal variation between 20 and 40 units rTA. These findings appear similar to those reported in cutaneous melanoma,20 45although, owing to the different methods used, the results between the two studies are not directly comparable. According to previous studies in the literature,45-52 a correlation between rTA and cell type, tumour grade or growth fraction appears to be dependent upon the type of tumour. In the current study, the telomerase expression or rTA of uveal melanomas did not correlate with either morphological (location, cell type) or immunohistological features (growth fraction, determined using the MIB-1 antibody) of the tumour. These findings are in agreement with those of Ogoshi et al, who investigated cutaneous melanomas.45 They contrast, however, with those observations of authors investigating neoplasms of the brain,46-49 thyroid,50lung,51 and the colon.52 Previous studies suggest that telomerase activity may have a role as a prognostic factor in some malignant neoplasms,52 as a correlation between the telomerase activity of the tumour cells and the outcome of the patients was observed. Although the number of uveal melanomas examined in the current study is small and the follow up period short, it appears that the amount of rTA does not have any prognostic implication in these tumours.
In vitro data have suggested that a gene located on chromosome 3 acts as a repressor gene of telomerase activity and, thereby, induces senescence in normal cells through the mitotic shortening of telomeres.27 53 54 Interestingly, recent studies have demonstrated certain genetic changes associated with uveal melanomas, in particular, monosomy 3, multiplication of chromosome 8q, loss of 9p, as well as the translocation t(3;22)(p13;p11).33-55Further, monosomy 3 in these tumours correlates with a worse prognosis.56 Because of this association, we also investigated the genotypic status of chromosome 3 in six of the tumours to determine any relation between monosomy 3 and telomerase activity. Only one of the uveal melanomas demonstrated a genotype −3,+8q, the remaining tumours were disomic for chromosome 3 (results not shown). These preliminary results would suggest that there is no influence of chromosome 3 status on either telomerase expression or activity in uveal melanoma. However, an investigation of a larger number of tumours is required for confirmation of this observation.
In the non-tumorous parts of the enucleated eyes, moderate to high expression of hTR was demonstrated by in situ hybridisation in the pre-equatorial and equatorial lens epithelium—that is, in the germinative zone of the lens. The hTR signal diminished gradually, however, in those elongated cells being transformed into lens fibres, closer to the lens nucleus. This finding is in agreement with previous studies demonstrating maximal mitotic activity in the germinative zone,57 indicating that these cells have a high replicative capacity. Although we did not determine telomerase activity in the lens, the strong hTR signals over the lens epithelium of the germinative zone would suggest the necessity of telomerase in these cells to maintain chromosomal stability. They could be compared, therefore, with physiologically proliferating cells such as lymphocytes and basal epithelial cells, where telomerase activity has been reported.16-20 Whether there is effect of age on telomerase in the lens epithelium—and whether there may be a relation with telomerase and the development of cataract—remains to be determined.
The telomere hypothesis proposes that differentiated non-dividing cells no longer require telomerase expression. High telomerase activity, for example, was observed in the brain in the embryo and neonate in rats, diminishing rapidly after birth.58 In addition, the human brain has been demonstrated to have no telomerase activity and to have long stable telomeres throughout adult life, consistent with their postmitotic status.59 Although we cannot completely exclude the possibility of false negative results, the absence of telomerase expression in most parts of the developed adult eye in the current study (see Results) would support the telomere hypothesis. As the retina represents a highly modified extension of the central nervous system, our findings of signals over scattered ganglion cells in the in situ hybridisation for hTR, however, contrast with those of the above mentioned investigations examining brain tissue. Maitraet al 60 also observed hTR upregulation in normal ganglion cells after birth as well as in the ganglion cells of four ganglioneuroblastomas and four ganglioneuromas. Despite this, these authors could not demonstrate telomerase activity in the ganglioneuromas examined using the TRAP assay,60confirming that the demonstration of hTR alone in cells does not prove telomerase activity in all cases.22 The biological relevance of persistent hTR expression in postmitotic somatic cells, such as the ganglion cells of the brain and retina, is unknown at present. The possibility that the signals over the ganglion cells in the current investigation represent artefacts cannot be completely excluded. The fact, however, that others60 using different probes also demonstrated hTR upregulation in ganglion cells located in the peripheral nervous system and that our sense probe was persistently negative would suggest that the hTR signals over the retinal ganglion cells are specific.
In conclusion, telomerase upregulation is a constant feature in uveal melanomas contributing to the malignant phenotype of these tumours. The amount of telomerase activity in uveal melanomas does not seem to have a prognostic influence on the outcome of this disease. Further, the expression of hTR could be demonstrated in both neoplastic and non-neoplastic parts of the eyes enucleated for uveal melanoma.
This work was supported by the Deutsche Forschungsgemeinschaft (DFG; ltu 557/2-1).
The authors thank Mrs Elisabeth Oker and Mrs Helga Zimmermann-Höffken for their supportive technical assistance. In addition, the authors are grateful for the examination of some of the uveal melanomas for aberrations of chromosome 3 by Dr. Dieter Lohmann, Institute of Human Genetics, Essen, and for his useful discussions.
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