Both CD4+ and CD8+ T cells are involved in protection against HSV-1 induced corneal scarring
- aOphthalmology Research, Cedars-Sinai Burns and Allen Research Institute, Los Angeles, USA, bDepartment of Ophthalmology, UCLA School of Medicine, Los Angeles, USA
- Homayon Ghiasi, Ophthalmology Research, Davis Building Room 5069, Cedars-Sinai Medical Center, 8700 Beverly Blvd, Los Angeles, CA 90048, USA
- Accepted 15 November 1999
AIM To determine the relative impact of CD4+ T cells and CD8+ T cells in protecting mice against ocular HSV-1 challenge.
METHODS CD4+ T cell knockout mice (CD4−/− mice), CD8+ T cell knockout mice (CD8−/− mice), and mice depleted for CD4+ or CD8+ T cells by antibody (CD4+ depleted and CD8+ depleted mice), were examined for their ability to withstand HSV-1 ocular challenge. The parental mice for both knockout mice were C57BL/6J.
RESULTS These results suggest that: (1) both CD4+ deficient mice (CD4−/− and CD4+ depleted mice) and CD8+ deficient mice (CD8−/−, and CD8+ depleted mice) developed significantly more corneal scarring than their C57BL/6J parental strain; (2) the duration of virus clearance from the eyes of the CD4+ deficient mice was 4 days longer than that of the CD8+ deficient mice; and (3) the severity of corneal scarring in the CD4+ deficient mice was approximately twice that of the CD8+ deficient mice.
CONCLUSIONS It was reported here that: (1) CD4+ and CD8+ T cells were both involved in protection against lethal ocular HSV-1 infection; and (2) CD4+ and CD8+ T cells were both involved in protection against HSV-1 induced corneal scarring.