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Br J Ophthalmol 84:523-526 doi:10.1136/bjo.84.5.523
  • Original Article
    • Laboratory science

Investigation of β defensin gene expression in the ocular anterior segment by semiquantitative RT-PCR

Table 2

  Tissue sample qualitative and semiquantitative PCR results obtained from the 23 tissue samples

Sample type hBD-1 hBD-2 hBD-1/GAPDH
Mean (SEM) (SD) 		hBD-2/GAPDH
Mean (SEM) (SD)
Cornea 5/5 3/5 0.166 (0.084) (0.187) 0.353 (0.292) (0.653)
Conjunctiva 5/6 2/6 0.568 (0.290) (0.709) 0.013 ( - ) (0.018)
Iris 5/6 0/6 0.232 (0.116) (0.285) —
Lens capsule 6/6 0/6 0.093 (0.056) (0.137) —

  • ↵Refers to the number of samples from which the two human β defensins were amplified.

  • ↵Refers to the human β defensin mRNA levels expressed as a ratio to the levels of the housekeeping gene (GAPDH).

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