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Br J Ophthalmol 85:1416-1420 doi:10.1136/bjo.85.12.1416
  • Scientific correspondence

Effect of three different media on serum free culture of donor corneas and isolated human corneal endothelial cells

Table 1

Endothelial cell densities at the beginning of organ culture and after 1, 2, and 3 weeks of culture in medium MEM, F99, and endothelial-SFM (SFM) with or without serum supplementation. Indicated are the means (SD) of the corneal endothelial cell densities of seven cornea

Medium Before organ culture After 7 days of organ culture After 14 days of culture After 21 days of culture
MEM without FCS 2360 (470) 1920 (350) 1880 (250) 1370 (630)
MEM + 2% FCS 2190 (590) 1960 (450) 1590 (430) 1590 (470)
F99 without FCS 2390 (310) 2020 (340) 1970 (330) 1770 (370)
F99 + 2% FCS 2260 (380) 1790 (520) 1720 (420) 1640 (470)
SFM without FCS 2600 (210) 1960 (420) 2040 (280) 1940 (200)
SFM + 2% FCS 2510 (340) 1930 (340) 2030 (230) 1840 (380)

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