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Br J Ophthalmol 85:732-738 doi:10.1136/bjo.85.6.732
  • Original Article
    • Laboratory science

A comparison of ocular melanocyte and uveal melanoma cell invasion and the implication of α1β1, α4β1 and α6β1 integrins

Table 4

Expression of integrins as detected by immunohistochemistry in a series of 10 cultured primary posterior uveal melanomas, and relation to in vitro cell morphology

Tumour Passage α1β1 α2β1 α3β1 α4β1 α5β1 α6β1 αVβ3 β1 In vitro cell type
Mel 204 5 h-50/++ +++ +/++ +/++ +++ +/++ + + epithelioid
Mel 205 3 h-50/++ h-60/+++ h-70/+ ++/+++ +/++ ++/+++ + epithelioid
Mel 206 4 ++/+++ + ++ h-30/+ +++ ++ ++/+++ + epithelioid
Mel 209 4 h-65/++ +/++ ++ h-50/+ + ++ +/++ + epithelioid
Mel 211 3 + ++/+++ ++/+++ ++ +++ −ve +/++ +/++ spindle
Mel 212 3 h-20/+ ++ ++ h-20/+ h-60/+/++ −ve ++ +++ mixed
Mel 213 3 +++ ++/+++ ++/+++ + ++ ++ +/++ + epithelioid
Mel 214 3 +++ + ++/+++ h-60/+ +++ ++ ++ ++ epithelioid
Mel 218 3 + + ++/+++ −ve ++ −ve ++ + mixed
Mel 222 4 h-70/+ +/++ +++ −ve + −ve h-70/+ ++ spindle

  • The percentage of cells staining was considered homogeneous if 75% of cells were stained. The expression was considered to be heterogeneous if the percentage of cells staining was between 5% and 75%, and the relevant percentage is indicated (h5–h75). Less than 5% of cells stained was considered negative. Staining intensity was scored as + = weak, ++ = medium, or +++ = strong.

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