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Br J Ophthalmol 2001;85:872-876 doi:10.1136/bjo.85.7.872
  • Original Article
    • Laboratory science

Induction of interleukin-8 in human retinal pigment epithelial cells after denuding injury

  1. Ayako Yoshidaa,
  2. Susan G Elnera,
  3. Zong-Mei Biana,
  4. Victor M Elnera,b
  1. aDepartment of Ophthalmology, University of Michigan, Ann Arbor, MI, USA, bDepartment of Pathology
  1. Victor M Elner, MD, PhD, Department of Ophthalmology, WK Kellogg Eye Center, University of Michigan, 1000 Wall Street, Ann Arbor, MI 48105, USAvelner{at}umich.edu
  • Accepted 8 February 2001

Abstract

AIM To determine interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) expression in response to mechanical injury in human retinal pigment epithelial (HRPE) cells.

METHODS Enzyme linked immunosorbent assay (ELISA) was performed to determine IL-8 and MCP-1 secretion by HRPE cells after mechanical denudation. IL-8 and MCP-1 mRNA expression by HRPE cells was assessed using semiquantitative RT-PCR. The effects of immunosuppressive drugs, dexamethasone (DEX) and cyclosporin A (CSA), as well as immunosuppressive cytokines, interleukin 4 (IL-4), interleukin 10 (IL-10), and interleukin 13 (IL-13), on chemokine expression in HRPE cells after denuding injury were analysed.

RESULTS Mechanical injury induced HRPE IL-8 mRNA and IL-8 secretion. Although MCP-1 mRNA was enhanced slightly after denuding injury, MCP-1 secretion was not increased. DEX and CSA inhibited HRPE chemokine expression after injury. IL-4 and IL-13 enhanced IL-8 and MCP-1 production by HRPE cells after injury while IL-10 had no effect.

CONCLUSIONS These results suggest that IL-8 may be involved in retinal inflammatory responses to injury and that DEX and/or CSA treatment may help control the inflammatory components of retinal diseases such as proliferative vitreoretinopathy.

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