You are here

Article Text

Article menu
Download PDFPDF
Scientific correspondence
Amniotic membrane grafts, “fresh” or frozen? A clinical and in vitro comparison
  1. Philip J Addsa,
  2. Charles J Huntb,
  3. John K G Dartc
  1. aDepartment of Biomedical Sciences, Queen Mary Westfield College, Mile End, London E1 4NS, UK, bTissue Services London and SE, East Anglia Tissue Bank, Long Road, Cambridge CB2 2PT, UK, cMoorfields Eye Hospital, City Road, London EC1V 2PD, UK
  1. Philip Adds, The Medical College of St Bartholomews and the Royal London, Department of Biomedical Sciences, Queen Mary Westfield College, Mile End, London E1 4NS, UKp.j.adds{at}qmw.ac.uk

Abstract

BACKGROUND/AIMS The use of “fresh” (hypothermically stored) and frozen amniotic membrane (AM) was compared in a patient with cicatricial pemphigoid with stem cell failure. The viability of both “fresh” and frozen AM epithelial cells was assessed after storage.

METHODS AM was stored at either +4°C (“fresh”) or at −80°C (frozen). A “fresh” graft was applied to the cornea following superficial keratectomy. Subsequently, a further frozen graft was applied to the same eye. Viability of the stored AM epithelium was assessed by investigating membrane integrity and mitochondrial activity.

RESULTS In both cases the cornea re-epithelialised and visual acuity improved. Improvement, however, was not sustained.

CONCLUSION Although both procedures led to an improvement in visual acuity, “fresh” tissue performed no better than frozen in promoting re-epithelialisation. The authors suggest that logistical, safety, and cost considerations outweigh any benefits of using “fresh” as opposed to frozen graft material.

  • amnion
  • corneal grafting
  • hypothermic preservation

https://doi.org/10.1136/bjo.85.8.905

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    View Full Text

    Footnotes