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Br J Ophthalmol 86:191-195 doi:10.1136/bjo.86.2.191
  • Original Article
    • Clinical science

Rapid mutation detection by the Transgenomic wave analyser DHPLC identifies MYOC mutations in patients with ocular hypertension and/or open angle glaucoma

Table 2

PCR primer sequences

Fragment Forward primer (name, sequence 5′–3′) Reverse primer (name, sequence 5′–3′) Size
Exon 1 MYOCe1.L ATCCAGGCACCTCTCAGCAC MYOCe1.R GCGCCTGTAGCAGGTCACTA 733 bp
Exon 2 MYOCe2.L AACATAGTCAATCCTTGGGC MYOCe2.R TTCTGTTCCTCTTCTCCTCC 341 bp
Exon 3 MYOCe3.L TCACATCTACTGGCTCTGCC MYOCe3.R CTGGCTGGCTGGCTCTCCCT 972 bp
Exon 3A MYOCe3.L3 GTCCGCCAGGTTTTTGAGTA MYOCe3.R3 GGTGCCACAGATGATGAAGG 423 bp
Exon 3B MYOCe3.L4 GTGCCATTGTCCTCTCCAAA MYOCe3.R4 CTCTCCCTTCAGCCTGCTC 395 bp
MY5 MY5.L TCTCTGTCCCTGCTACGTCTT MY5.R GCACCCATACCCCAATAATAG ∼239 bp
MY3 MY3.L GTTGGGAGATGTGATTGCAG MY3.R AGATGGAGGTGGGAAAGTGT ∼145 bp

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