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Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique
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  • Published on:
    Author Reply: Dynamics of corneal endothelial death in organ culture
    • Gilles Thuret, Ophthalmologist
    • Other Contributors:
      • Christophe Chiquet, Lydia Campos, and Philippe Gain

    Dear Editor

    We read with interest the remarks of Crowston et al. [1] on our article entitled "Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique".[2] We showed that the TUNEL technique revealed a far higher percentage of endothelial cells (ECs) irreversibly engaged in a cell death process than that obtained by trypan blue staining.

    ...

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    Conflict of Interest:
    None declared.
  • Published on:
    Cell death assays for endothelial viability in corneal donor tissue

    Dear Editor

    We read with interest the paper by Gain et al. [1] which assessed two distinct techniques to quantify corneal endothelial cell death in donor corneas. A significantly higher rate of cell death was observed with the TUNEL assay which labels nuclei with fragmented DNA, compared to the trypan blue exclusion method, which detects cells with disrupted cell membranes. The authors conclude that...

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    Conflict of Interest:
    None declared.