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Scientific correspondence
S antigen specific effector T cell activation detected by cytokine flow cytometry
  1. J P Morgan1,
  2. R A Robins2,
  3. H S Dua3,
  4. P J Tighe4
  1. 1The Larry Donoso Research Laboratory, Division of Ophthalmology and Visual Sciences, Eye Ear Nose and Throat Centre, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK
  2. 2Department of Immunology, University of Nottingham, UK
  3. 3The Larry Donoso Research Laboratory, Division of Ophthalmology and Visual Sciences, University of Nottingham, UK
  4. 4Departments of Ophthalmology and Immunology, University of Nottingham, UK
  1. Correspondence to: James P Morgan; James.Morgan{at}nottingham.ac.uk

Abstract

Background/aims: Effector T cell activation is particularly important in the initiation of autoimmune uveitis. This pilot study seeks to demonstrate activation of human peripheral effector T cells in response to the uveitis candidate autoantigen, retinal S antigen (SAg), using cytokine flow cytometry (CFC).

Methods: Peripheral blood mononuclear cell (PBMC) suspensions from uveitis patients and controls were stimulated with bovine SAg. Activation responses were detected by CFC.

Results: Electronic gating enabled analysis of CD69+, IFN-γ+ CD4+ lymphocytes. An SAg specific response was detectable in four of 13 patients and four of eight controls.

Conclusion: SAg specific, peripheral, effector T cell activation can be detected by CFC. Similar levels of responsiveness were seen in patient and control groups. More detailed cytokine profiling may demonstrate functional differences between the groups.

  • uveoretinitis
  • cytokine
  • flow cytometry
  • S antigen
  • CFC, cytokine flow cytometry
  • EAU, experimental autoimmune uveoretinitis
  • PBMC, peripheral blood mononuclear cell
  • PCR, polymerase chain reaction
  • PMA, phorbol myristate acetate
  • SAg, retinal S antigen

https://doi.org/10.1136/bjo.86.5.517

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