Table 1
IL-10, IL-12, TNFα levels in explant culture supernatants, with and without IL-10 neutralisation
| Culture conditions | IL-10 | IL-12 | TNFα |
|---|---|---|---|
| Data represented are mean (SEM) of cytokine levels obtained after 48 hours of culture from a minimum of three experiments. Culture conditions include medium alone, IFNγ/LPS (activated), TGFβ, IFNγ/LPS + TGFβ, with and without anti IL-10 mAb. (Cytokine concentrations are pg/ml). Statistical comparisons were made following one way analysis of variance with an F test to allow multiple comparisons. | |||
| *IL-10 in medium alone versus activated; p = 0.0053. | |||
| †IL-12 in medium alone versus TGFβ; p = 0.016, IL-12 in medium alone versus TGFβ (+anti IL-10); p = 0.03. | |||
| ‡TNFα in medium alone versus activated; p = 0.029, TNFα in medium alone versus activated (+anti-IL-10); p = 0.003. | |||
| Medium alone | |||
| − anti IL-10 mAb | 16.7 (10.3)* | 327.9 (45.4)† | 14.5 (4.4)‡ |
| + anti IL-10 mAb | 4.8 (4.1) | 489.7 (314.1) | 196.1 (111.6) |
| Activated | |||
| − anti IL-10 mAb | 167.0 (54.4)* | 150.8 (42.4)† | 57.5 (12.2)‡ |
| + anti IL-10 mAb | 3.1 (2.7) | 211.7 (211.7) | 273.0 (138.8) |
| TGFβ | |||
| − anti IL-10 mAb | 44.44 (12.1) | 95.6 (66.3) | 65 (30) |
| + anti IL-10 mAb | 4.3 (3.7) | 86.3 (99) | 240.4 (284.7) |
| Activated + TGFβ | |||
| − anti IL-10 mAb | 197.7 (39.9) | 13.6 (10.6) | 84.9 (15.4) |
| + anti IL-10 mAb | 15.6 (13.4) | 42.9 (42.9) | 227.0 (169.0) |
