Immunohistochemical staining of lasered and control retinas
| Specimen number | Origin of specimen | Laser before enucleation (days) | NT4 | GFAP | CD68 |
|---|---|---|---|---|---|
| Intensity of staining: +++ = strong, ++ = moderate, + = mild, − = negative. *Staining reduced in outer nuclear layer at the site of the laser burn, †staining of inner and outer nuclear layers of the retina, ‡staining throughout the whole retina, §scattered staining of cells predominantly in the nerve fibre layer. | |||||
| 1 | Melanoma | 1 | +* | +++‡ | + |
| 2 | Melanoma | 2 | +* | +++‡ | + |
| 3 | Melanoma | 6 | +* | +++‡ | ++ |
| 4 | Melanoma | 6 | +* | +++‡ | ++ |
| 5 | Melanoma | NA | +† | +++‡ | − |
| 6 | Melanoma | NA | +† | +++‡ | − |
| 7 | Melanoma | NA | − | +++‡ | − |
| 8 | Cadaveric donor | NA | ++† | +§ | − |
| 9 | Cadaveric donor | NA | ++† | +§ | − |
| 10 | Cadaveric donor | NA | ++† | +§ | − |









