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Letter
Propionibacterium acnes endophthalmitis diagnosed by microdissection and PCR
  1. R R Buggage1,
  2. D F Shen1,
  3. C-C Chan1,
  4. D G Callanan2
  1. 1Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, USA
  2. 2Texas Retina Associates, Arlington, TX, USA
  1. Correspondence to: Ronald R Buggage, MD, NIH/NEI, Building 10, Room 10N112, Bethesda, MD 20892-1857, USA; buggager{at}nei.nih.gov

https://doi.org/10.1136/bjo.87.9.1190

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    Although Propionibacterium acnes, a Gram positive anaerobic bacillus, is the most commonly identified cause of delayed onset postoperative endophthalmitis, routine vitreous cultures are frequently inadequate for its diagnosis. This case describes the utility of the histopathological technique of microdissection and polymerase chain reaction (PCR) for the diagnosis of delayed postoperative endophthalmitis.

    Case report

    A 78 year old man with a history of vitreous floaters, a coronary bypass, and aortic valve replacement underwent an uncomplicated cataract extraction with intraocular lens (IOL) implantation in the right eye. Three months later, he developed increasing floaters in the right eye and was diagnosed with vitritis unresponsive to corticosteroid treatment. Examination revealed acuities of 20/25 in the right eye and 20/20 in the left with normal intraocular pressures. The right eye was significant for no anterior chamber cells or flare, dilated iris vessels, an IOL without deposits, 3+ vitreous cells with trace haze, and peripheral pigmentary degeneration. The left eye was normal with the exception …

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      CORRECTIONS
      BMJ Publishing Group Ltd.BMA House, Tavistock Square, London, WC1H 9JR
      British Journal of Ophthalmology 2003; 87 1432-1432 Published Online First: 10 Nov 2003. doi: 10.1136/bjo.87.11.1432-b