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Br J Ophthalmol 88:393-398 doi:10.1136/bjo.2003.018481
  • Clinical science
    • Extended reports

Epithelial cell characteristics of cultured human limbal explants

Table 2

Immunohistochemical characteristics of the basal, suprabasal, and superficial cells of the explants at 1 and 3 weeks

Day 0 Week 1 Week3
Antibody specificity B SB S B SB S B SB S
B = basal; SB = suprabasal; S = superficial.
α Enolase and epidermal growth factor receptor showed homogeneous staining throughout the limbal explant at all time points. No immunoreactivity was observed in the limbal epithelial cells for CD34 or for the neuroendocrine, Langerhans cells, or melanocyte markers at any of the time points.
Cytokeratin 3 Negative Negative Many cells positive Negative Negative Few cells positive Negative Negative Occasional cell positive
Cytokeratin 19 Few cells positive Negative Negative Positive Positive Positive Positive Positive Positive
Vimentin Positive Few cells positive Negative Positive Positive Positive Positive Positive Positive
Connexin43 Occasional cell positive Positive Negative Negative Negative Negative Negative Negative Negative
Ki67 Negative Negative Negative Many cells positive Many cells positive Occasional cell positive Occasional cell positive Negative Negative
P63 Many cells positive Occasional cell positive Negative Many cells positive Occasional cell positive Negative Occasional cell positive Negative Negative

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