Immunohistochemical characteristics of the basal, suprabasal, and superficial cells of the explants at 1 and 3 weeks
| Day 0 | Week 1 | Week3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Antibody specificity | B | SB | S | B | SB | S | B | SB | S |
| B = basal; SB = suprabasal; S = superficial. | |||||||||
| α Enolase and epidermal growth factor receptor showed homogeneous staining throughout the limbal explant at all time points. No immunoreactivity was observed in the limbal epithelial cells for CD34 or for the neuroendocrine, Langerhans cells, or melanocyte markers at any of the time points. | |||||||||
| Cytokeratin 3 | Negative | Negative | Many cells positive | Negative | Negative | Few cells positive | Negative | Negative | Occasional cell positive |
| Cytokeratin 19 | Few cells positive | Negative | Negative | Positive | Positive | Positive | Positive | Positive | Positive |
| Vimentin | Positive | Few cells positive | Negative | Positive | Positive | Positive | Positive | Positive | Positive |
| Connexin43 | Occasional cell positive | Positive | Negative | Negative | Negative | Negative | Negative | Negative | Negative |
| Ki67 | Negative | Negative | Negative | Many cells positive | Many cells positive | Occasional cell positive | Occasional cell positive | Negative | Negative |
| P63 | Many cells positive | Occasional cell positive | Negative | Many cells positive | Occasional cell positive | Negative | Occasional cell positive | Negative | Negative |









