T lymphocyte mediated lysis of mitomycin C treated Tenon’s capsule fibroblasts
- 1Wound Healing Research Unit, Institute of Ophthalmology, Bath Street, London EC1V 9EL, UK
- 2Glaucoma Unit, Moorfields Eye Hospital, City Road, London EC1V 2PD, UK
- 3Department of Clinical Immunology, Royal Free Hospital School of Medicine, Pond Street, London NW3, UK
- Correspondence to: J G Crowston Hamilton Glaucoma Center, UCSD, 9500 Gilman Drive, La Jolla, CA 92093-0946, USA; jcrowstonucsd.edu
- Accepted 25 June 2003
Abstract
Aims: To evaluate the effect of T cell co-culture on mitomycin C treated and untreated Tenon’s capsule fibroblasts.
Methods: IL-2 dependent allogeneic T cells were incubated over a monolayer of mitomycin C treated or control fibroblasts. Fibroblast numbers were evaluated by direct counts using phase contrast microscopy. To determine whether T cell mediated lysis was a consequence of MHC mismatch, co-culture experiments were repeated with autologous T cells. The effect of Fas receptor blockade was established by co-incubation with a Fas blocking (M3) antibody.
Results: T cell co-culture resulted in a dramatic reduction in fibroblast survival compared to mitomycin C treatment alone (p = 0.032). T cell killing required fibroblast/lymphocyte cell to cell contact and was observed in both allogeneic and autologous co-culture experiments. Fas blocking antibodies did not significantly inhibit T cell killing (p = 0.39).
Conclusion: T cells augment mitomycin C treated fibroblast death in vitro. Similar mechanisms may contribute to the cytotoxic effect of mitomycin C in vivo and account for the largely hypocellular drainage blebs that are observed clinically.
