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Br J Ophthalmol 88:579-581 doi:10.1136/bjo.2003.022020
  • Letter

Controlled study of the influence of storage medium type on endothelial assessment during corneal organ culture

Table 3

Final examination of paired corneas stored in medium A and B

Group A (n = 30) Group B (n = 30) p Value
Results were expressed as mean (standard deviation), minimum–maximum/median. The automatic analysis was less relevant at delivery than at receipt (see Table 1), consequently, a touch up analysis should be recommended.
Image quality (good/average/poor, (%)) 0/23/77 43/43/14 <0.001
Cells well recognised per se, “automatic” mode (n) 159 (47) 75–248/159 238 (46) 151–324/238 <0.001
ECD, “automatic” mode (cells/mm2) 3143 (159) 2829–3543/3105 3029 (188) 2650–3451/3039 0.011
Cells counted, “touch up” mode (n) 357 (72) 209–559/339 456 (82) 292–608/446 <0.001
ECD, “touch up” mode (cells/mm2) 2708 (320) 2277–3430/2704 2716 (284) 2259–3579/2686 0.934
Overall cell loss, “touch up” mode (%) 12.6 (9.3) 7.2–34.1/12.2 13.1 (8.0) 1.3–27.8/15.6 0.79
Coefficient of variation of cell area, “touch up” mode (%) 28.4 (3.5) 23.7–40.4/27.8 27.2 (3.4) 21.7–34.8/27.3 0.098
Hexagonality, “touch up” mode (%) 50.7 (9.2) 32.5–72.8/49.7 53.0 (6.6) 36.2–66.6/52.6 0.201
Touch up duration (seconds) 551 (134) 361–853/521 388 (120) 226–653/362 <0.001

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