Trypan blue: authors’ reply
- The Royal Liverpool University Hospital, Prescot Street, Liverpool L7 8XP, UK
- Correspondence to: DrKenneth K Li Prince of Wales Hospital, Shatin, NT, Hong Kong; vitreousrcsed.ac.uk
- Accepted 25 September 2003
We would like to thank Dr Rodrigues and colleagues for bringing up this interesting point of what exactly trypan blue stains.1
In our study, immunohistochemistry was performed to determine the nature of cells involved in the epiretinal membranes (ERM)—not to determine the presence or absence of the ERM. Presence or absence of ERM was determined by examining routinely stained sections (haematoxylin and eosin, periodic acid Schiff) for cytoplasm/nuclei of epiretinal cell elements. All four …
