Table 2
L-selectin detection and lymphocyte adhesion in subjects with diabetes and controls
| Method | Controls | Diabetics | p v control |
|---|---|---|---|
| Results of surface L-selectin on CD3+ cells detected by flow cytometry (% positive cells), nuclear mRNA detected by RT-PCR (L-selectin: β actin ratio), shed L-selectin in the serum (ng/ml) detected by ELISA and lymphocyte adhesion to a HUVEC monolayer (% of control value, which has arbitrarily been set at 100%) are tabled for patients with diabetes and controls. Mean values (SD) are indicated. | |||
| L-selectin on CD3+(FACS) | 74.17% (6.51) (n = 17) | 63.94% (11.34) (n = 36) | 0.004 |
| L-selectin mRNA (RT-PCR) | 1.246 (0.7) (n = 7) | 1.086 (0.303) (n = 13) | 0.8 |
| Serum L-selectin (ELISA) | 828 ng/ml (177) (n = 12) | 987 ng/ml (243) (n = 28) | 0.06 |
| Lymphocyte adhesion | 100% (n = 7) | 144% (65) (n = 9) | 0.1 |
