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Br J Ophthalmol 2004;88:1206-1208 doi:10.1136/bjo.2004.042101
  • Laboratory science - Scientific reports

Histopathological analysis in experimental macular surgery with trypan blue

  1. S Grisanti1,
  2. P Szurman1,
  3. O Tatar1,
  4. F Gelisken1,
  5. S Aisenbrey2,
  6. J Oficjalska-Mlynczak3,
  7. R Kaczmarek1,
  8. K U Bartz-Schmidt1
  1. 1Department of Ophthalmology I, Eberhard-Karls University Tuebingen, Germany
  2. 2Department of Neuroscience, Tufts University, Boston, MA, USA
  3. 3Department of Ophthalmology, University of Wroclaw, Poland
  1. Correspondence to: Assistant professor S Grisanti Department of Ophthalmology I, Eberhard-Karls University of Tuebingen, Schleichstrasse 12–15, 72076 Tuebingen, Germany; salvatore.grisantimed.uni-tuebingen.de
  • Accepted 29 February 2004

Abstract

Aim: To analyse the effect of trypan blue on the retina in an experimental setting of macular surgery.

Methods: Porcine eyes were used within 3 hours after death. The eyes were hemisected and the vitreous removed. Trypan blue (0.15%) was applied over the trephined posterior pole, whereas the rest of the eye cup was filled with a balanced salt solution (BSS). The dye and the BSS were removed after 1 minute and the complete eye cup irrigated and filled with fresh BSS. Both the treated and untreated retinas were illuminated with a standard surgical light pipe and source at maximum power for 10 minutes. Both the trypan blue exposed retina and the non-treated surrounding retina were processed for histology.

Results: Exposure of the retina to trypan blue for 1 minute, followed by illumination caused no histologically detectable damage compared to the controls. No microarchitectural disorganisation, cellular disruption, or affection of the vitreoretinal interface was detected.

Conclusions: These findings indicate that a 1 minute exposure of trypan blue followed by illumination does not cause an acute morphologically detectable toxic effect on the porcine retina.

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