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Br J Ophthalmol 89:1510-1517 doi:10.1136/bjo.2005.072108
  • Laboratory science - Extended reports

The expression of native and cultured human retinal pigment epithelial cells grown in different culture conditions

Table 7

 Differential gene expression between cultured and native RPE by real time RT-PCR

Gene name GenBank Acc No Fold change, array* Fold change, RT-PCR* p Value
*Cultured RPE/native RPE; †0, undetectable in subconfluent ARPE-19 cells; ‡∞, undetectable in native RPE,
Subconfluent v native RPE
SEC13-like 1 (S cerevisiae) AA496784 0† −12.5 0.040
ATPase, H+ transporting, lysosomal V0 subunit a isoform 1 AA427472 −3.6 −1135 0.039
Topoisomerase (DNA) II α170 kDa AA504348 16.9 ∞‡
Confluent, serum withdrawn v native RPE
START domain containing 4, sterol regulated H11369 −2.5 −5.7 0.0012
Oxidase (cytochrome c) assembly 1-like AA598582 10.5 2.71 0.0032
Topoisomerase (DNA) II α170 kDa AA504348 17.4 ∞‡

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