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Br J Ophthalmol 89:904-910 doi:10.1136/bjo.2004.056218
  • Laboratory science - Extended reports

TLRs and NODs mRNA expression pattern in healthy mouse eye

  1. S Rodríguez-Martínez1,3,
  2. M E Cancino-Díaz2,
  3. L Jiménez-Zamudio2,
  4. E García-Latorre2,
  5. J C Cancino-Díaz1
  1. 1Laboratorio de Microbiología General, Departamento de Microbiología de la Escuela Nacional de Ciencias Biológicas del Instituto Politecnico Nacional, Carpio y Plan de Ayala, México DF 11340, México
  2. 2Laboratorio de Inmunoquímica I, Departamento de Inmunología de la Escuela Nacional de Ciencias Biológicas del Instituto Politecnico Nacional, Carpio y Plan de Ayala, México DF 11340, México
  3. 3Laboratorio de Inmunología Ocular del Instituto de Oftalmología, Fundación Conde de Valenciana, México DF, México
  1. Correspondence to: Dr Juan Carlos Cancino-Díaz Departamento de Microbiología, Laboratorio de Microbiología General, Escuela Nacional de Ciencias Biológicas, Carpio y Plan de Ayala s/n, México, DF, 11340, México; jccancinodiazhotmail.com
  • Accepted 1 December 2004

Abstract

Aims: To look for TLR and NOD mRNA expression in the healthy eye and in other immune privileged and non-immune privileged mouse organs.

Methods: Semiquantitative RT-PCR was performed to look for TLR1–9 and NOD1 and NOD2 mRNA expressions in the whole eye, in the anterior (AP) and posterior (PP) portions of the eye, in corneal fibroblasts (CF) and in ovary, brain, testis, heart, lung, and spleen.

Results: All the TLR mRNAs were expressed in the whole eye of Balb/c mice. NIH and C57BL/6 did not express TLR9 and TLR8, respectively. NIH expressed higher levels of TLR1, 2, 3, and 6 than the other strains. C57BL/6 expressed the lowest levels of all TLRs. TLR9, 5, and 4 were the less expressed in all strains. All TLRs were expressed in Balb/c PP and TLR1 was not expressed in AP. In NIH and Balb/c CF the majority of TLRs were overexpressed with LPS. In testis, expression of most TLRs was absent. Non-immune privileged organs expressed most of the TLRs. All the organs expressed NOD1 and NOD2. In PP NOD2 was not expressed.

Conclusion: TLRs and NODs are expressed in the eye, and could have an important role in the innate immunity.

Footnotes